Guo Qingxiang, Jia Luo, Qin Jianhua, Li Hong, Gu Zemao
1 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University , Wuhan, China .
2 Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan, China .
Foodborne Pathog Dis. 2015 Aug;12(8):653-60. doi: 10.1089/fpd.2014.1903. Epub 2015 Jun 4.
Myxobolus honghuensis parasitizes the pharynx of the allogynogenetic gibel carp Carassius auratus gibelio (Bloch), an economically important food fish with large consumption rates in China. The fact that it is hard to detect the cysts formed by M. honghuensis without euthanizing the fish and the spores can reach the consumers has prompted us to investigate the effect of M. honghuensis on conventional, immunocompetent BALB/c suckling mice, in order to consider the possible pathological effects in general immunocompetent consumers by the ingestion of Myxobolus-infected fish. BALB/c suckling mice were inoculated with serial dilutions of the spore suspension and then hematological and histological studies were performed. Tests of suckling mice showed no statistically difference of fluid accumulation ratio between mice inoculated with 1.65 × 10(6) M. honghuensis spores and negative control after 8-h incubation. Spores did not disrupt intestinal histology, and no abnormal bowel movements were observed within 20 h postinoculation. No anomalous hematology parameters were documented. These results, taken together, reveal that M. honghuensis spores do not cause pathology (diarrhea and elevated fluid accumulation) in BALB/c suckling mice. However, an extrapolation is not currently feasible because of lack of a highly quantifiable risk extrapolation model, inaccessibility to data for the effect of M. honghuensis on humans, and lack of consideration of the immunosuppressed population. Our study extends the results of the effect of myxozoan on suckling mice and serves as baseline for future experiment using the same model in evaluating the myxozoan-caused foodborne disease.
洪湖碘泡虫寄生于异育银鲫（Carassius auratus gibelio (Bloch)）的咽部，异育银鲫是一种在中国消费量很大的重要经济食用鱼类。由于不处死鱼就很难检测到洪湖碘泡虫形成的囊肿，而且孢子可能会进入消费者体内，这促使我们研究洪湖碘泡虫对常规的、具有免疫能力的BALB/c乳鼠的影响，以便考虑食用感染碘泡虫的鱼对一般具有免疫能力的消费者可能产生的病理影响。给BALB/c乳鼠接种系列稀释的孢子悬液，然后进行血液学和组织学研究。对乳鼠的测试表明，接种1.65×10(6)个洪湖碘泡虫孢子的小鼠与阴性对照组在孵育8小时后的积液率没有统计学差异。孢子没有破坏肠道组织学结构，接种后20小时内未观察到异常的肠道运动。没有记录到异常的血液学参数。综合这些结果表明，洪湖碘泡虫孢子不会在BALB/c乳鼠中引起病理变化（腹泻和积液增加）。然而，由于缺乏高度可量化的风险外推模型、无法获取洪湖碘泡虫对人类影响的数据以及未考虑免疫抑制人群，目前进行外推是不可行的。我们的研究扩展了粘孢子虫对乳鼠影响的结果，并为未来使用相同模型评估粘孢子虫引起的食源性疾病的实验提供了基线。
