Trägårdh Johanna, Robb Gillian, Gadalla Kamal K E, Cobb Stuart, Travis Christopher, Oppo Gian-Luca, McConnell Gail
Opt Lett. 2015 Aug 1;40(15):3484-7. doi: 10.1364/OL.40.003484.
We have developed a simple wavelength-tunable optical parametric generator (OPG), emitting broadband ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third-harmonic generation, of nuclei of brain cells and blood vessels in a >150 μm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW.
我们开发了一种简单的波长可调谐光学参量发生器（OPG），用于非线性无标记显微镜检查，它能发射峰值波长在1530 - 1790 nm的宽带超短脉冲。该OPG由一块周期极化铌酸锂晶体组成，由具有高脉冲能量的超快Yb：光纤激光器在1064 nm波长下泵浦。我们证明，这种OPG可用于对厚度大于150μm的脑组织切片中的脑细胞和血管细胞核进行无标记成像，通过三次谐波产生实现，成像深度处强度衰减极小，在15 mW的入射平均功率下对组织无可见损伤。
