Hebda Lisa M, Foran David R
Forensic Science Program, School of Criminal Justice, Michigan State University, 655 Auditorium Road, 560A Baker Hall, East Lansing, MI, 48824.
Department of Integrated Biology, Michigan State University, East Lansing, MI, 48824.
J Forensic Sci. 2015 Sep;60(5):1322-30. doi: 10.1111/1556-4029.12878. Epub 2015 Aug 10.
DNA identification of human remains is often necessary when decedents are skeletonized; however, poor DNA recovery and polymerase chain reaction (PCR) inhibition are frequently encountered, a situation exacerbated by burial. In this research, the utility of integrating soil DNA isolation kits into buried skeletal DNA analysis was evaluated and compared to a standard human DNA extraction kit and organic extraction. The soil kits successfully extracted skeletal DNA at quantities similar to standard methods, although the two kits tested, which differ mechanistically, were not equivalent. Further, the PCR inhibitors calcium and humic acid were effectively removed using the soil kits, whereas collagen was less so. Finally, concordant control region sequences were obtained from human skeletal remains using all four methods. Based on these comparisons, soil DNA isolation kits, which quickened the extraction process, proved to be a viable extraction technique for skeletal remains that resulted in positive identification of a decedent.
当死者只剩骨架时，通常需要进行人类遗骸的DNA鉴定；然而，经常会遇到DNA回收不佳和聚合酶链反应（PCR）抑制的情况，而埋葬会加剧这种情况。在本研究中，评估了将土壤DNA提取试剂盒整合到埋葬骨骼DNA分析中的效用，并与标准人类DNA提取试剂盒和有机提取方法进行了比较。土壤试剂盒成功提取出的骨骼DNA数量与标准方法相似，尽管测试的两种试剂盒在机制上有所不同，并不等效。此外，使用土壤试剂盒可有效去除PCR抑制剂钙和腐殖酸，而对胶原蛋白的去除效果则较差。最后，使用所有四种方法均从人类骨骼遗骸中获得了一致的控制区序列。基于这些比较，加快提取过程的土壤DNA提取试剂盒被证明是一种可行的骨骼遗骸提取技术，可用于对死者进行阳性鉴定。
