PEI-RGD/125I-(αv)反义寡脱氧核苷酸对肝癌细胞HepG2生长和侵袭的抑制作用

Inhibitory Effects of PEI-RGD/125I-(αv) ASODN on Growth and Invasion of HepG2 Cells.

作者信息

Cai Haidong, Qiao Yu, Sun Ming, Yuan Xueyu, Luo Qiong, Yang Yuehua, Yuan Shidong, Lv Zhongwei

机构信息

Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).

Department of Blood Transfusion, Changhai Hospital, Second Military Medical University, Shanghai, China (mainland).

出版信息

Med Sci Monit. 2015 Aug 10;21:2339-44. doi: 10.12659/MSM.893973.

DOI:10.12659/MSM.893973

PMID:26258995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4536871/

Abstract

BACKGROUND

To investigate the in vitro inhibitory effects of PEI-RGD/125I-(αv)ASODN (PEI, polyethylenimine; RGD, Arg-Gly-Asp; ASODN, antisense oligodeoxynucleotide) on the growth and invasion of HepG2 cells.

MATERIAL AND METHODS

ASODN of the integrin αv-subunit was marked with 125I and underwent complexation with PEI-RGD, a PEI derivative. Next, PEI-RGD/125I-(αv) ASODN was introduced into HepG2 cells via receptor-mediated transfection, and its inhibition rate on HepG2 cell growth was tested using the methyl thiazolyl tetrazolium (MTT) method. The effects of PEI-RGD/125I-(αv) ASODN on HepG2 cell invasion ability were evaluated using the Boyden chamber assay.

RESULTS

The 125I marking rate of (αv) ASODN was 73.78±4.09%, and the radiochemical purity was 96.68±1.38% (greater than 90% even after a 48-h incubation period at 37°C), indicating high stability. 2) The cytotoxicity assays showed that the cell inhibition rates did not differ significantly between the PEI-RGD/125I-(αv)ASODN group and the PEI-RGD/(αv) ASODN group, but they were both significantly higher than in the other groups and were positively correlated (r=0.879) with the dosage within a certain range. 3) The invasion assays showed that the inhibition rate was significantly greater in the PEI-RGD/125I-(αv) ASODN group compared to the other groups.

CONCLUSIONS

PEI-RGD/125I-(αv) ASODN can efficiently inhibit the growth and proliferation of HepG2 cells and can also weaken their invasive ability.

摘要

背景

研究聚乙烯亚胺-精氨酸-甘氨酸-天冬氨酸/125I-（αv）反义寡脱氧核苷酸（PEI，聚乙烯亚胺；RGD，精氨酸-甘氨酸-天冬氨酸；ASODN，反义寡脱氧核苷酸）对肝癌细胞系HepG2生长和侵袭的体外抑制作用。

材料与方法

将整合素αv亚基的反义寡脱氧核苷酸用125I标记，并与聚乙烯亚胺衍生物PEI-RGD进行络合。然后，通过受体介导的转染将PEI-RGD/125I-（αv）ASODN导入HepG2细胞，并用噻唑蓝（MTT）法检测其对HepG2细胞生长的抑制率。采用Boyden小室法评估PEI-RGD/125I-（αv）ASODN对HepG2细胞侵袭能力的影响。

结果

1）（αv）ASODN的125I标记率为73.78±4.09%，放射化学纯度为96.68±1.38%（即使在37℃孵育48小时后仍大于90%），表明稳定性高。2）细胞毒性试验表明，PEI-RGD/125I-（αv）ASODN组与PEI-RGD/（αv）ASODN组的细胞抑制率差异无统计学意义，但均显著高于其他组，且在一定范围内与剂量呈正相关（r=0.879）。3）侵袭试验表明，PEI-RGD/125I-（αv）ASODN组的抑制率显著高于其他组。