Department of Stomatology, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 170120-901, Brazil.
Department of Community Dentistry, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Clin Oral Investig. 2016 May;20(4):745-52. doi: 10.1007/s00784-015-1557-6. Epub 2015 Aug 13.
The aim of this study was to investigate the relationship the expression of cytokeratins (CK10 and CK13) and the cell proliferation index determined by Ki-67 of lip squamous cell carcinoma and actinic cheilitis with different degrees of dysplasia.
Forty-five paraffin-embedded actinic cheilitis with and without dysplasia and 20 lip squamous cell carcinoma were analyzed by immunohistochemistry using anti-human anti-CK10, anti-CK13, and anti-Ki-67 antibodies.
The majority of actinic cheilitis showed immunopositivity for CK10 and CK13 with decrease or loss of expression in dysplastic areas. In lip squamous cell carcinoma of the lip, heterogeneous expression of CK13 and immunonegativity for CK10 were observed. There was a statistically significant difference between CK10 expression in lip squamous cell carcinoma and in actinic cheilitis with or without dysplasia (p < 0.001). The cell proliferation index was higher in actinic cheilitis with dysplasia and lip squamous cell carcinoma than in actinic cheilitis without epithelial dysplasia. A significant correlation was found between the intensity of the epithelial dysplasia and the cell proliferation index (p < 0.001).
These results provide evidence that there is a downregulation of CK10 expression in dysplastic areas of patients with actinic cheilitis and in those with lip squamous cell carcinoma (LSCC) and that the index of cell proliferation, determined by Ki-67, is directly correlated with the intensity of the epithelial dysplasia.
Altogether, these results suggest that CK10 expression and the epithelial cell proliferation index can help to identify malignant transformation in the lip region.
本研究旨在探讨细胞角蛋白（CK10 和 CK13）的表达与 Ki-67 细胞增殖指数在不同程度不典型增生的光化性唇炎和唇鳞状细胞癌中的关系。
采用免疫组织化学法检测 45 例伴或不伴不典型增生的光化性唇炎和 20 例唇鳞状细胞癌石蜡包埋组织中抗人 CK10、CK13 和 Ki-67 抗体的表达。
大多数光化性唇炎表现为 CK10 和 CK13 的免疫阳性，在不典型增生区域表达减少或缺失。在唇鳞状细胞癌中，观察到 CK13 的异质性表达和 CK10 的免疫阴性。CK10 在唇鳞状细胞癌和有或无不典型增生的光化性唇炎中的表达有统计学差异（p<0.001）。有不典型增生的光化性唇炎和唇鳞状细胞癌的细胞增殖指数高于无上皮不典型增生的光化性唇炎。上皮不典型增生的严重程度与细胞增殖指数之间存在显著相关性（p<0.001）。
这些结果表明，在光化性唇炎和唇鳞状细胞癌的不典型增生区域中 CK10 表达下调，Ki-67 确定的细胞增殖指数与上皮不典型增生的严重程度直接相关。
综上所述，这些结果表明 CK10 表达和上皮细胞增殖指数有助于识别唇部的恶性转化。
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