Department of Oral and Maxillofacial Pathology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, São Paulo, SP, 05508-000, Brazil.
Eur Arch Otorhinolaryngol. 2018 Jul;275(7):1877-1883. doi: 10.1007/s00405-018-5002-8. Epub 2018 May 19.
The aim of this work was to evaluate the expression of the cancer stem cell (CSC) markers CD44, ALDH1 and p75NTR in the ultraviolet-induced lesions actinic cheilitis (AC) and lip squamous cell carcinoma (LSCC), and to correlate it with p53 expression.
Immunohistochemistry was performed in 4 cases of normal lip (NL), 43 of AC and 20 of LSCC.
All cases were positive for CD44, showing a membranous staining without differences between the groups. ALDH1 showed cytoplasmic staining and it was invariable amongst the grades of epithelial dysplasia and between AC and LSCC. p75NTR presented membranous/cytoplasmic staining in the basal and parabasal layer of NL and AC, while LSCC presented cytoplasmic staining in the peripheral layers of the tumor islands. p75NTR showed different expression amongst the dysplasia grades (p < 0.001) but no differences between AC and LSCC. p53 expression was similar amongst the dysplasia grades and between AC and LSCC. CD44, ALDH1 and p75NTR were unrelated amongst themselves and to p53 expression.
CSC markers are expressed in potentially malignant and malignant lesions of the lip. Their expressions were invariable between AC and LSCC and unrelated to p53. p75NTR expression increased with the worsening of epithelial dysplasia grade.
本研究旨在评估癌症干细胞（CSC）标志物 CD44、ALDH1 和 p75NTR 在紫外线诱导的光化性唇炎（AC）和唇鳞状细胞癌（LSCC）病变中的表达，并将其与 p53 表达相关联。
对 4 例正常唇（NL）、43 例 AC 和 20 例 LSCC 进行免疫组织化学染色。
所有病例均为 CD44 阳性，各组之间无差异，呈膜染色。ALDH1 呈细胞质染色，在上皮异型增生程度和 AC 与 LSCC 之间无差异。p75NTR 在 NL 和 AC 的基底和副基底层呈膜/细胞质染色，而 LSCC 的肿瘤岛周边层呈细胞质染色。p75NTR 在异型增生程度之间的表达不同（p<0.001），但在 AC 和 LSCC 之间无差异。p53 表达在异型增生程度和 AC 与 LSCC 之间相似。CD44、ALDH1 和 p75NTR 之间彼此无关，与 p53 表达也无关。
CSC 标志物在唇的潜在恶性和恶性病变中表达。它们在 AC 和 LSCC 之间的表达不变，与 p53 无关。p75NTR 的表达随着上皮异型增生程度的恶化而增加。
