人类抗体对巴西利什曼原虫巴西亚种抗原的特异性分析。
Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis.
作者信息
Masuda A, do Nascimento S F, Guerra C S, Paranhos G da S, Ferreira A W
出版信息
Rev Inst Med Trop Sao Paulo. 1989 Jul-Aug;31(4):228-34. doi: 10.1590/s0036-46651989000400004.
The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b. braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.
用1%脱氧胆酸钠在10 mM Tris-Hcl pH 8.2中处理巴西利什曼原虫（L. b. braziliensis）前鞭毛体，通过免疫印迹法分析其抗原性，使用来自美洲皮肤利什曼病（ACL）、美洲内脏利什曼病（AVL）、血吸虫病、疟疾和恰加斯病患者的血清作为探针。ACL血清始终与一条60 kD的条带发生反应。上述其他疾病的血清对该蛋白未观察到反应性，表明60 kD蛋白可用于ACL的血清诊断。
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