在兔实验性眼部炎症中产生的是前列腺素E2而非E1。

Production of prostaglandin E2 rather than E1 in experimental ocular inflammation of rabbit.

作者信息

Tanouchi Y, Yokota K, Yamamoto S, Mimura Y

出版信息

Tokushima J Exp Med. 1989 Jun;36(1-2):47-52.

Abstract

The chopped anterior uvea of rabbit was allowed to react with exogenous [1-14C] arachidonic acid or prostaglandin (PG)H2. Several cyclooxygenase products such as PGD2, PGE2 and PGF2 alpha were produced. In the presence of glutathione PGE2 was a major product. When uveitis was induced by injection of bovine serum albumin into the vitreous body, there was a marked invasion of leukocytes. PGE2 in the aqueous humor increased about 3-fold as determined by radioimmunoassay using anti-PGE2 antibody cross-reacting with PGE1. Extracts from the inflamed aqueous humor and the incubation medium of chopped anterior uvea or peripheral polymorphonuclear leukocytes were analyzed by reverse-phase high-performance liquid chromatography, which allowed separation of PGE2 and PGE1. In all these preparations PGE2 was an almost sole immunoreactive PG, and PGE1 was hardly detectable in sharp contrast to an earlier report (Eakins et al.: Nature 239: 248 (1972].

摘要

将兔切碎的前葡萄膜与外源性[1-¹⁴C]花生四烯酸或前列腺素（PG）H₂反应。产生了几种环氧化酶产物，如PGD₂、PGE₂和PGF₂α。在谷胱甘肽存在的情况下，PGE₂是主要产物。当通过向玻璃体注射牛血清白蛋白诱导葡萄膜炎时，有明显的白细胞浸润。使用与PGE₁交叉反应的抗PGE₂抗体通过放射免疫测定法测定，房水中的PGE₂增加了约3倍。通过反相高效液相色谱法分析来自发炎房水以及切碎的前葡萄膜或外周多形核白细胞孵育培养基的提取物，该方法可以分离PGE₂和PGE₁。在所有这些制剂中，PGE₂几乎是唯一的免疫反应性PG，与早期报告（伊金斯等人：《自然》239：248（1972年））形成鲜明对比的是，几乎检测不到PGE₁。