Kaplan J E, Saba T M
Adv Shock Res. 1979;2:83-92.
Reticuloendothelial system (RES) depression has been correlated with diminished resistance to trauma, shock, and sepsis in man and animals. Previous studies have related the depression of RES hepatic Kupffer cell phagocytic function after trauma to diminished bioassayable opsonic activity. The present study determined if the loss of biological activity and RES alteration correlated with immunoreactive serum opsonic alpha 2 SB glycoprotein levels after trauma. Serum opsonic activity was measured by liver slice bioassay, and immunoreactive opsonic protein was measured by rocket electroimmunoassay. RE function was determined by colloid clearance over a 24-hour post-trauma period. Anesthetized rats (250-300 gm) subjected to sublethal or severe (greater than LD50) whole-body NCD trauma were the shock models investigated. Immunoreactive levels in 63 rats prior to injury were 518 +/- 24 microgram/ml. Neither biological nor immunoreactive levels were altered over 24 hours in anesthetized sham-traumatized controls. Temporal alteration in the initial decrease and recovery pattern of biologically active and immunoreactive opsonic protein levels significantly correlated following both sublethal and severe injury. Moreover, the patterns of immunoreactive levels of the opsonic protein correlated with the functional phagocytic activity of the RES as determined by vascular clearance of a test dose of blood-borne radiolabeled particulates. This glycoprotein falls after trauma, and the magnitude and duration of the decline increases with severity of injury. Immunoreactive opsonic alpha 2 SB glycoprotein appears to be an accurate measurement of circulating opsonic activity and RE Kupffer cell function after trauma, especially with respect to clearance. Thus, immunoreactive opsonic protein warrants clinical consideration as a noninvasive measure of reticuloendothelial systemic defense in patients after trauma and burn.
网状内皮系统（RES）功能抑制与人和动物对创伤、休克及败血症的抵抗力下降有关。先前的研究已将创伤后RES肝库普弗细胞吞噬功能的抑制与生物测定的调理活性降低联系起来。本研究确定创伤后生物活性丧失和RES改变是否与免疫反应性血清调理α2 SB糖蛋白水平相关。通过肝切片生物测定法测量血清调理活性，通过火箭免疫电泳法测量免疫反应性调理蛋白。通过创伤后24小时的胶体清除率来确定RE功能。以接受亚致死性或严重（大于LD50）全身非控制性出血创伤的麻醉大鼠（250 - 300克）作为研究的休克模型。63只大鼠在受伤前的免疫反应性水平为518±24微克/毫升。在麻醉的假创伤对照中，生物活性和免疫反应性水平在24小时内均未改变。在亚致死性和严重损伤后，生物活性和免疫反应性调理蛋白水平的初始下降和恢复模式的时间变化显著相关。此外，调理蛋白免疫反应性水平的模式与通过测试剂量的血源性放射性标记颗粒的血管清除率所确定的RES功能性吞噬活性相关。这种糖蛋白在创伤后下降，下降的幅度和持续时间随损伤严重程度增加。免疫反应性调理α2 SB糖蛋白似乎是创伤后循环调理活性和RE库普弗细胞功能的准确测量指标，尤其是在清除方面。因此，免疫反应性调理蛋白作为创伤和烧伤患者网状内皮系统防御的非侵入性测量指标值得临床考虑。
