[慢性病毒性肝炎的免疫遗传学与进展]

[IMMUNOGENETICS AND PROGRESSION OF CHRONIC VIRAL HEPATITES].

作者信息

Beloborodova E V, Goncharova I A, Beloborodova E I, Purlik I L, Kalacheva T P, Akbasheva O E, Rachkovskiy M I, Burkovskaya V A

出版信息

Eksp Klin Gastroenterol. 2015(3):45-9.

Abstract

AIM OF INVESTIGATION

To estimate the role of polymorphic variants of genes IL4 (C-590T), IL4RA (I50V), TNF (G-308A) and SLC1IAI (D543N) in chronic viral hepatitis progression.

MATERIAL AND METHODS

Overall 121 patients with chronic viral hepatitis C and B.

RESULTS

Study results have demonstrated, that of all investigated polymorphic variants of genes IL4 (C-590T), IL4RA (150V), TNF (G-308A) and SLC11A1 (D543N) in patients with chronic liver diseases of various etiology the <<Val/Val>> IL4RA gene variant (II50Val) is associated with HBV-infection chronization. The allele <> gene TNF-α (G-308A) is protective for chronic viral hepatitis and is associated with a low level of production by mononuclear cells of TNF-α and IL-12, high secretion of IL-4 and low degree of collagenopathy in the liver. Genotype <> of IL4 (C-590T) gene is an adverse marker for progression of chronic viral hepatitis B. There was significant correlation of polymorphic variants TNF (G-308A) and IL4 (C-590T) genes with production of the key interleukins, that determine type of immune response (Th-1, Th-2) and products of collagen metabolism that testifies genetic determination of system immune response and collagen formation processes in the liver at chronic viral hepatitis.

研究目的

评估白细胞介素4（IL4，C-590T）、白细胞介素4受体α（IL4RA，I50V）、肿瘤坏死因子（TNF，G-308A）和溶质载体家族11成员1（SLC11A1，D543N）基因多态性变异在慢性病毒性肝炎进展中的作用。

材料与方法

共纳入121例慢性丙型和乙型病毒性肝炎患者。

结果

研究结果表明，在各种病因的慢性肝病患者中，所有检测的IL4（C-590T）、IL4RA（I50V）、TNF（G-308A）和SLC11A1（D543N）基因多态性变异中，IL4RA基因的<<Val/Val>>变异体（I50Val）与乙肝病毒感染慢性化有关。TNF-α（G-308A）基因的<>等位基因对慢性病毒性肝炎具有保护作用，且与单核细胞产生低水平的TNF-α和IL-12、高水平的IL-4分泌以及肝脏中低程度的胶原病有关。IL4（C-590T）基因的<>基因型是慢性乙型病毒性肝炎进展的不良标志物。TNF（G-308A）和IL4（C-590T）基因的多态性变异与关键白细胞介素的产生显著相关，这些白细胞介素决定免疫反应类型（Th-1、Th-2）以及胶原代谢产物，这证明了慢性病毒性肝炎时肝脏系统免疫反应和胶原形成过程的遗传决定性。