[狗肝菜多糖P1A的结构特征研究]

[Studies on structure characteristic of polysaccharide P1A from Dicliptera chinensis].

作者信息

He Yu-yuan, Xie Lu-wei, Du Guan-feng, Zhan Jiang-cheng, Yin Yong-qin, Shen Zhi-bin

出版信息

Zhongguo Zhong Yao Za Zhi. 2015 Apr;40(8):1489-92.

Abstract

The chemical structures of P1 A was identified by complete acid hydrolysis, partial acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, IR and NMR. The results showed that P1 A had a backbone consisting rhamnose, mannose, glucose and galactose. The side chain possessed arabinose and xylose. 1-->, 1-->6 and non-reducing terminal linkages existed in polysaccharide P1A, but there are doubling amount of 1-->2 and 1-->4 linkages. Oxidable linkage of P1 A accounted for 45%, and inoxidable linkage of P1A accounted for 55%. Mannose, glucose and galactose were mainly linked by 1-->2 linkage. Rhamnose, arabinose and xylose were mainly linked by 1-->2 and 1-->4 linkages. PlA contained beta-Glc(1,6)-,beta-Gal(1,3)-,beta-Man(1,4)-beta-Rha,-Glc(1,4)-, Glc(1)-,-Gal(1,4)- and Man(1)-.

摘要

通过完全酸水解、部分酸水解、高碘酸氧化-史密斯降解、甲基化分析、红外光谱和核磁共振对P1 A的化学结构进行了鉴定。结果表明，P1 A具有由鼠李糖、甘露糖、葡萄糖和半乳糖组成的主链。侧链含有阿拉伯糖和木糖。多糖P1A中存在1→、1→6和非还原末端连接，但1→2和1→4连接的数量翻倍。P1 A的可氧化连接占45%，不可氧化连接占55%。甘露糖、葡萄糖和半乳糖主要通过1→2连接。鼠李糖、阿拉伯糖和木糖主要通过1→2和1→4连接。P1A含有β-Glc(1,6)-、β-Gal(1,3)-、β-Man(1,4)-β-Rha、-Glc(1,4)-、Glc(1)-、-Gal(1,4)-和Man(1)-。