Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, China.
Carbohydr Res. 2013 Jan 10;365:20-5. doi: 10.1016/j.carres.2012.10.013. Epub 2012 Oct 24.
A complex polysaccharide, termed LRP4-A, was isolated from the fruit of Lycium ruthenicum Murr. and its structure was characterized. The crude polysaccharide LRP was obtained from the fruit of L. ruthenicum Murr. using hot water extraction followed by ethanol precipitation. The water-soluble polysaccharide LRP4-A was purified from LRP by anion-exchange chromatography and gel filtration chromatography. Its molecular weight was 1.05×10(5) Da. Monosaccharide composition analysis revealed that LRP4-A mainly consisted of rhamnose, arabinose, glucose, and galactose in the molar ratio of 1:7.6:0.5:8.6, with a trace of xylose. Structure of the polysaccharide LRP4-A was characterized using a series of analytical techniques, including methylation analysis, partial acid hydrolysis, IR, NMR, and ESI-MS. LRP4-A was identified to be a highly branching polysaccharide with a backbone of β-(1→6)-linked galactose partially substituted at O-3 position. The branches were composed of (1→3)-linked-Gal, (1→3)-linked-Ara, (1→5)-linked-Ara, and (1→2,4)-linked-Rha. Arabinose, galactose, and glucose were located at the termini of the branches.
从黑果枸杞的果实中分离得到一种复杂的多糖，命名为 LRP4-A，并对其结构进行了表征。粗多糖 LRP 采用热水提取、乙醇沉淀法从黑果枸杞果实中提取得到。将水溶性多糖 LRP4-A 从 LRP 中通过阴离子交换色谱和凝胶过滤色谱进行纯化。其分子量为 1.05×10(5) Da。单糖组成分析表明，LRP4-A 主要由鼠李糖、阿拉伯糖、葡萄糖和半乳糖组成，摩尔比为 1:7.6:0.5:8.6，还有微量的木糖。采用一系列分析技术，包括甲基化分析、部分酸水解、IR、NMR 和 ESI-MS，对 LRP4-A 的结构进行了表征。LRP4-A 被鉴定为一种高度支化的多糖，其骨架由β-(1→6)-连接的半乳糖组成，部分在 O-3 位取代。支链由(1→3)-连接的-Gal、(1→3)-连接的-Ara、(1→5)-连接的-Ara 和(1→2,4)-连接的-Rha 组成。阿拉伯糖、半乳糖和葡萄糖位于支链的末端。
