土曲霉阿魏酸酯酶A的表达及其在生物质降解中的应用。
Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation.
作者信息
Zhang Shuai-Bing, Wang Le, Liu Yan, Zhai Huan-Chen, Cai Jing-Ping, Hu Yuan-Sen
机构信息
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
出版信息
Protein Expr Purif. 2015 Nov;115:153-7. doi: 10.1016/j.pep.2015.08.015. Epub 2015 Aug 14.
Feruloyl esterases (FAEs) are key enzymes involved in the complete biodegradation of lignocelluloses, which could hydrolyze the ester bonds between hemicellulose and lignin. The coding sequence of a feruloyl esterase A (AtFaeA) was cloned from Aspergillus terreus and the recombinant AtFaeA was constitutively expressed in Pichia pastoris. The SDS-PAGE analysis of purified AtFaeA showed two protein bands owing to the different extent of glycosylation, and the recombinant AtFaeA had an optimum temperature of 50°C and an optimum pH of 5.0. The substrate utilization and primary sequence identity of AtFaeA demonstrated that it is a type-A feruloyl esterase. The hydrolysis of corn stalk and corncob by xylanase from Aspergillus niger could be significantly improved in concert with recombinant AfFaeA.
阿魏酸酯酶（FAEs）是参与木质纤维素完全生物降解的关键酶，它能够水解半纤维素和木质素之间的酯键。从土曲霉中克隆了阿魏酸酯酶A（AtFaeA）的编码序列，并在毕赤酵母中组成型表达重组AtFaeA。纯化后的AtFaeA经SDS-PAGE分析显示出两条蛋白带，这是由于糖基化程度不同所致，且重组AtFaeA的最适温度为50°C，最适pH为5.0。AtFaeA的底物利用情况和一级序列一致性表明它是一种A型阿魏酸酯酶。黑曲霉木聚糖酶对玉米秸秆和玉米芯的水解作用可与重组AfFaeA协同显著提高。
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