Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Int J Biol Macromol. 2010 May 1;46(4):440-4. doi: 10.1016/j.ijbiomac.2010.01.020. Epub 2010 Feb 6.
This paper describes cloning, expression, characterization and crystallization of feruloyl esterase from Aspergillus awamori. The gene coding for feruloyl esterase from A. awamori was amplified, cloned, and expressed in Escherichia coli. The recombinant enzyme was purified, characterized and used for crystallization. Its X-ray crystallographic data were collected and explained using molecular replacement theory. Also, the tertiary structure of the recombinant enzyme-ferulic acid complex was also determined.
本文描述了 Aspergillus awamori 中木酰基酯酶的克隆、表达、特性和结晶。从 Aspergillus awamori 中扩增、克隆和表达了编码木酰基酯酶的基因。重组酶经纯化、鉴定后用于结晶。利用分子置换理论对其 X 射线晶体学数据进行了收集和解释。此外，还确定了重组酶-阿魏酸复合物的三级结构。
