Ayaz M, Baba F, Akgun N, Bas A L, Uney K, Dik B
Bratisl Lek Listy. 2015;116(7):451-6. doi: 10.4149/bll_2015_085.
The current study aims to investigate the possible role of NO distillate either for therapeutic or for protective potential in diabetic cardiomyopathy.
Protective and restorative effects of distillated Nerium oleander (NO) on the diabetes-induced electrophysiological and structural alterations were investigated.
Type 2 diabetes was induced by combination of single dose streptozotocin injection and high fat diet for four weeks. Experimental groups were designed as follows: control, diabetic, restorative-NO treated diabetic and protective-NO treated diabetic. Intracellular action potentials (AP) and contractile activities were measured form left ventricular papillary muscle strips as well as histopathological examination of heart tissue and biochemical examinations of serum were performed.
Type 2 diabetes induced AP prolongation was prevented with both ways of NO treatments. Moreover, treatments produced nearly complete restorations of diabetes-induced depressed amplitude and altered kinetics of contractile activities. In parallel to electrophysiological parameters, both histopathological and biochemical results indicates the NO induced beneficial effects on the diabetes related alterations.
Distillated Nerium oleander (NO) can be a highly potential therapeutic or preventive agent on the diabetes induced excitation-contraction coupling alterations (Tab. 3, Fig. 3, Ref. 23).
本研究旨在探讨夹竹桃提取物（NO）在糖尿病性心肌病中可能的治疗或保护作用。
研究了夹竹桃提取物（NO）对糖尿病引起的电生理和结构改变的保护及恢复作用。
通过单次注射链脲佐菌素并结合高脂饮食四周诱导2型糖尿病。实验组设计如下：对照组、糖尿病组、恢复性NO治疗糖尿病组和保护性NO治疗糖尿病组。测量左心室乳头肌条的细胞内动作电位（AP）和收缩活动，并对心脏组织进行组织病理学检查以及对血清进行生化检查。
两种NO治疗方式均能防止2型糖尿病诱导的AP延长。此外，治疗使糖尿病导致的收缩活动振幅降低和动力学改变几乎完全恢复。与电生理参数一致，组织病理学和生化结果均表明NO对糖尿病相关改变具有有益作用。
夹竹桃提取物（NO）可能是治疗或预防糖尿病诱导的兴奋 - 收缩偶联改变的极具潜力的药物（表3，图3，参考文献23）。
