Al-Turki T A, Basahi Mohammed A
Natural Resources and Environmental Research Institute, King Abdulaziz City for Science and Technology, P.O. 6086, Riyadh 11442, Saudi Arabia.
Shaqra University, College of Science and Arts Sajir, P.O. Box 33, Shaqra 11961, Saudi Arabia.
Saudi J Biol Sci. 2015 Sep;22(5):591-9. doi: 10.1016/j.sjbs.2015.06.027. Epub 2015 Jul 8.
Inter simple sequence repeat (ISSR) analysis, using 14 primers was performed to estimate genetic diversity among 27 landraces of Hassawi rice growing in Al-Ahsa region of Saudi Arabia and deposited at King Abdulaziz City for Science and Technology with KACST IDs. The average polymorphism produced by 11 selected primers was more than 75%. The analysis of ISSR polymorphism divided the examined rice landraces into two groups; In one group (A), one accession (KACST 191) was clearly delimited as a distant landrace from other 12 landraces grouped in two clusters; cluster I of seven landraces of close geographic distributions; four of them grow at close geographic locations (KACST IDs 32, 183, 184, 185, 186, 187 and 188) and cluster II is comprised of five landraces KACST IDs (190, 308, 352, 353 and 355). In group B, the landraces were more closely related to each other as compared to the landraces of group A. In this group a small cluster of two landraces (KACST 305 & KACST 333) was clearly distant from a large group of three clusters comprised of landraces having KACST IDs 189 & 192, landraces 302, 306, 307, 308 & 310 and landraces with KACST IDs 334, 351, 354, 356 & 357 respectively. These results indicate that ISSR fingerprints are efficient in the identification and resolution of genetic diversity between the landraces of the Hassawi rice and will be an efficient method in the authentication of the rice germplasm in the gene bank of Saudi Arabia.
利用14个引物进行了简单重复序列区间（ISSR）分析，以评估沙特阿拉伯哈萨地区种植的27个哈萨维水稻地方品种的遗传多样性，这些品种保存在阿卜杜勒阿齐兹国王科技城，并拥有KACST编号。11个选定引物产生的平均多态性超过75%。ISSR多态性分析将所检测的水稻地方品种分为两组；在一组（A组）中，一个种质（KACST 191）被明确界定为与其他12个地方品种距离较远，这12个地方品种又分为两个聚类；聚类I包含7个地理分布相近的地方品种；其中4个生长在相近地理位置（KACST编号32、183、184、185、186、187和188），聚类II由5个地方品种（KACST编号190、308、352、353和355）组成。在B组中，与A组的地方品种相比，这些地方品种之间的亲缘关系更为密切。在该组中，一小群两个地方品种（KACST 305和KACST 333）明显与一大群三个聚类距离较远，这三个聚类分别由具有KACST编号189和192的地方品种、302、306、307、308和310号地方品种以及分别具有KACST编号334、351、354、356和357的地方品种组成。这些结果表明，ISSR指纹图谱在鉴定和解析哈萨维水稻地方品种之间的遗传多样性方面是有效的，并且将成为沙特阿拉伯基因库中水稻种质鉴定的有效方法。
