Li Chun Lei, Seneviratne Chaminda Jayampath, Huo Lei, Lu Weijia William, Zheng Li Wu
Discipline of Oral Diagnosis & Polyclinics, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China; Department of Oral Medicine, Peking University School & Hospital of Stamotology, Beijing, China.
Discipline of Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore.
J Craniomaxillofac Surg. 2015 Oct;43(8):1662-9. doi: 10.1016/j.jcms.2015.07.001. Epub 2015 Jul 14.
Bisphosphonates-related osteonecrosis of the jaws (BRONJ) is a severe complication of BPs therapy with unknown pathogenesis. This study aimed to evaluate the impact of Actinomyces naeslundii (A. naeslundii) on the progression of BRONJ in ovariectomized (OVX) rat model with periodontal diseases. Sixty rats were randomly assigned into four groups. All rats underwent bilateral ovariectomy. Six weeks after surgery, animals with periodontitis induced by ligature placement were administrated with normal saline (NS), NS &A. naeslundii inoculation, zolecdronic acid (ZA) and ZA &A. naeslundii inoculation for 12 weeks, respectively. Loads of total bacteria and A. naeslundii in the mouth were assessed by real time PCR. After sacrifice, the mandibles were harvested for micro-computed tomography (micro-CT) and histological examination. Real-time PCR demonstrated that A. naeslundii was not routinely found in the rats and ZA treatment did not promote its accumulation. Micro-CT examination disclosed that ligature placement induced significant alveolar bone loss, which was greatly attenuated by ZA treatment and aggravated by A. naeslundii. Histological assessment demonstrated that ZA treatment increased the risk of developing BRONJ-like disease but this condition was not worsen with the presence of A. naeslundii. Our study suggested that oral A. naeslundii inoculation aggravated periodontal disease but not BRONJ in our animal model.
双膦酸盐相关颌骨坏死（BRONJ）是双膦酸盐治疗的一种严重并发症，其发病机制尚不清楚。本研究旨在评估内氏放线菌（A. naeslundii）对患有牙周疾病的去卵巢（OVX）大鼠模型中BRONJ进展的影响。60只大鼠被随机分为四组。所有大鼠均接受双侧卵巢切除术。术后6周，对通过结扎诱导牙周炎的动物分别给予生理盐水（NS）、NS与A. naeslundii接种、唑来膦酸（ZA）以及ZA与A. naeslundii接种，持续12周。通过实时PCR评估口腔中总细菌和A. naeslundii的载量。处死后，采集下颌骨进行微型计算机断层扫描（micro-CT）和组织学检查。实时PCR表明，大鼠中未常规发现A. naeslundii，且ZA治疗未促进其积累。Micro-CT检查显示，结扎导致显著的牙槽骨丢失，ZA治疗可大大减轻这种情况，而A. naeslundii则会加重这种情况。组织学评估表明，ZA治疗增加了发生类BRONJ疾病的风险，但在存在A. naeslundii的情况下，这种情况并未恶化。我们的研究表明，在我们的动物模型中，口服接种A. naeslundii会加重牙周疾病，但不会加重BRONJ。
