Li Wen-Zhong, Ai Zhi-Ying, Wang Zhi-Wei, Chen Lin-Lin, Guo Ze-Kun, Zhang Yong
College of Life Sciences, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China.
School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
Biochem Biophys Res Commun. 2015 Sep 25;465(3):575-9. doi: 10.1016/j.bbrc.2015.08.062. Epub 2015 Aug 18.
Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression.
Nanog维持小鼠胚胎干细胞（mESCs）的多能性。深入了解Nanog的调控对于更好地理解控制mESCs多能性的分子机制至关重要。在计算机分析中，我们在Nanog近端启动子中鉴定出四个GATA-1假定结合位点。启动子报告基因检测证明，异位表达GATA-1可显著抑制Nanog启动子活性。突变研究表明，四个假定结合位点之一对GATA-1抑制Nanog启动子活性起作用。电泳迁移率变动分析和染色质免疫沉淀证实了GATA-1与Nanog近端启动子的直接结合。我们的数据为协调Nanog表达的扩展调控网络提供了新见解。
