Joonè Carolynne J, De Cramer Kurt G M, Nöthling Johan O
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Onderstepoort, 0110, South Africa.
Rant en Dal Animal Hospital, 51 Cecil Knight Street, Mogale City, 1751, South Africa.
Reprod Fertil Dev. 2017 Feb;29(2):368-373. doi: 10.1071/RD15174.
Two full-term canine fetuses were found to share a placenta during Caesarean section. The fetuses were of discordant gender, with apparently normal male and female external genitalia. Genetic analysis of whole-blood samples obtained from each fetus revealed identical DNA profiles, with more than two alleles detected at six loci. Subsequent genetic analysis of myocardial tissue samples revealed dissimilar DNA profiles, with at most two alleles detected per locus. Superimposition of the tissue-derived profiles matched that derived from the blood samples exactly, except for two loci failing to amplify, and hence demonstrated blood chimaerism. Dissection of the abdomen of the male fetus revealed delayed descent of the testes towards the inguinal canals. Macroscopically, the gonads, uterus and vagina were not identifiable on dissection of the female fetus, although vestigial ovarian tissue and a vagina were detected microscopically. The hypoplastic internal reproductive tract of the female fetus was suggestive of freemartinism and is believed to be the first report of this condition in the canine.
在剖腹产手术中发现两只足月犬胎儿共用一个胎盘。这两个胎儿性别不同，雄性和雌性的外生殖器外观正常。对从每个胎儿采集的全血样本进行基因分析，结果显示DNA图谱相同，在六个位点检测到两个以上的等位基因。随后对心肌组织样本进行基因分析，结果显示DNA图谱不同，每个位点最多检测到两个等位基因。除了两个位点未能扩增外，组织来源的图谱与血液样本来源的图谱完全匹配，从而证实了血液嵌合体现象。解剖雄性胎儿的腹部发现睾丸向腹股沟管下降延迟。宏观上，解剖雌性胎儿时未发现性腺、子宫和阴道，但在显微镜下检测到残留的卵巢组织和阴道。雌性胎儿发育不全的内生殖管道提示为自由马丁现象，据信这是犬类中该病症的首例报道。
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