School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand.
School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand; Comvita NZ Limited, Wilson South Road, Paengaroa, PB1, Te Puke, New Zealand.
Food Chem. 2016 Feb 1;192:1006-14. doi: 10.1016/j.foodchem.2015.07.118. Epub 2015 Jul 26.
The fluorescence characteristics of various New Zealand honeys were investigated to establish if this technique might detect signatures unique to manuka (Leptospermum scoparium) and kanuka (Kunzea ericoides) honeys. We found unique fluorescence profiles for these honeys which distinguished them from other New Zealand honey floral types. Two excitation-emission (ex-em) marker wavelengths each for manuka and kanuka honeys were identified; manuka honey at 270-365 (MM1) and 330-470 (MM2) nm and kanuka honey at 275-305 (KM1) and 445-525 (KM2) nm. Dilution of manuka and kanuka honeys with other honey types that did not possess these fluorescence profiles resulted in a proportional reduction in fluorescence signal of the honeys at the marker wavelengths. By comparison, rewarewa (Knightia excelsa), kamahi (Weinmannia racemosa), and clover (Trifolium spp.) honeys did not exhibit unique fluorescence patterns. These findings suggests that a fluorescence-based screening approach has potential utility for determining the monoflorality status of manuka and kanuka honeys.
研究了各种新西兰蜂蜜的荧光特性,以确定该技术是否可以检测到麦卢卡(Leptospermum scoparium)和卡努卡(Kunzea ericoides)蜂蜜特有的特征。我们发现这些蜂蜜具有独特的荧光特征,可以将它们与其他新西兰花蜜类型区分开来。确定了两种麦卢卡和卡努卡蜂蜜的激发-发射(ex-em)标记波长;麦卢卡蜂蜜在 270-365(MM1)和 330-470(MM2)nm,卡努卡蜂蜜在 275-305(KM1)和 445-525(KM2)nm。将麦卢卡和卡努卡蜂蜜与不具有这些荧光特征的其他蜂蜜类型稀释,会导致蜂蜜在标记波长处的荧光信号成比例降低。相比之下,瑞瓦瓦(Knightia excelsa)、卡玛希(Weinmannia racemosa)和三叶草(Trifolium spp.)蜂蜜没有表现出独特的荧光模式。这些发现表明,基于荧光的筛选方法可能对确定麦卢卡和卡努卡蜂蜜的单花性状态具有潜在的用途。