School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand.
School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, PB92019 Auckland, New Zealand.
Food Chem. 2018 Nov 30;267:355-367. doi: 10.1016/j.foodchem.2017.07.065. Epub 2017 Jul 13.
New Zealand manuka (Leptospermum scoparium) and kanuka (Kunzea ericoides) honeys contain a unique array of chemical markers useful for chemical fingerprinting. We investigated the presence of 13 potential marker compounds in nectars of the major honey crop species. We confirmed that leptosperin, lepteridine, 2'-methoxyacetophenone, and 2-methoxybenzoic acid are exclusive to manuka nectar whereas lumichrome is unique to kanuka nectar. 3-Phenyllactic acid and 4-hydroxyphenyllactic acid are present in manuka and kanuka nectars. Leptosperin, lepteridine, 3-phenyllactic acid, and 4-hydroxyphenyllactic acid are chemically stable over prolonged storage, but not 2-methoxybenzoic acid and 2'-methoxyacetophenone. Accordingly, leptosperin and lepteridine are definitive chemical markers for authentication of manuka honey. An optimal concentration cut-off was established for the floral source-specific markers: leptosperin (94mg/kg), lepteridine (2.1mg/kg), 2'-methoxyacetophenone (2.0mg/kg) for manuka honey, and lumichrome (4.5mg/kg) for kanuka honey. The use of leptosperin and lepteridine as fluorescence markers for manuka honey authentication is reinforced.
新西兰麦卢卡（Leptospermum scoparium）和卡努卡（Kunzea ericoides）蜂蜜含有独特的一系列化学标志物，可用于化学指纹识别。我们研究了主要花蜜作物中 13 种潜在标记化合物的存在。我们证实，leptosperin、lepteridine、2'-甲氧基苯乙酮和 2-甲氧基苯甲酸是麦卢卡花蜜特有的，而光色氨酸是卡努卡花蜜特有的。3-苯乳酸和 4-羟基苯乳酸存在于麦卢卡和卡努卡花蜜中。leptosperin、lepteridine、3-苯乳酸和 4-羟基苯乳酸在长时间储存过程中化学性质稳定，但 2-甲氧基苯甲酸和 2'-甲氧基苯乙酮则不然。因此，leptosperin 和 lepteridine 是麦卢卡蜂蜜鉴定的明确化学标志物。建立了用于鉴定花蜜来源的特定标记物的最佳浓度截断值：麦卢卡蜂蜜中的 leptosperin（94mg/kg）、lepteridine（2.1mg/kg）和 2'-甲氧基苯乙酮（2.0mg/kg），卡努卡蜂蜜中的光色氨酸（4.5mg/kg）。麦卢卡蜂蜜认证中使用 leptosperin 和 lepteridine 作为荧光标记物的做法得到了加强。
