Fang Jinghua, Levchenko Igor, Mai-Prochnow Anne, Keidar Michael, Cvelbar Uros, Filipic Gregor, Han Zhao Jun, Ostrikov Kostya Ken
Plasma Nanoscience Laboratories, Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organisation (CSIRO), P.O. Box 218, Lindfield, NSW 2070, Australia.
School of Physics, University of Melbourne, Parkville, VIC, Australia, 3010.
Sci Rep. 2015 Aug 26;5:13379. doi: 10.1038/srep13379.
Dense arrays of gold-supported silver nanowires of about 100 nm in diameter grown directly in the channels of nanoporous aluminium oxide membrane were fabricated and tested as a novel platform for the immobilization and retention of BSA proteins in the microbial-protective environments. Additional treatment of the silver nanowires using low-temperature plasmas in the inductively-coupled plasma reactor and an atmospheric-pressure plasma jet have demonstrated that the morphology of the nanowire array can be controlled and the amount of the retained protein may be increased due to the plasma effect. A combination of the neutral gold sublayer with the antimicrobial properties of silver nanowires could significantly enhance the efficiency of the platforms used in various biotechnological processes.
制备了直径约100 nm、直接生长在纳米多孔氧化铝膜通道中的金负载银纳米线密集阵列,并将其作为一种新型平台,用于在微生物保护环境中固定和保留牛血清白蛋白(BSA)蛋白。在电感耦合等离子体反应器和大气压等离子体射流中使用低温等离子体对银纳米线进行额外处理,结果表明,由于等离子体效应,可以控制纳米线阵列的形态,并增加保留蛋白的量。具有抗菌特性的中性金底层与银纳米线相结合,可以显著提高用于各种生物技术过程的平台的效率。