通过流式细胞术对大鼠急性胰腺炎期间胰腺腺泡细胞中核因子κB激活进行定量分析。
Quantitation of nuclear factor kappa B activation in pancreatic acinar cells during rat acute pancreatitis by flow cytometry.
作者信息
Chen Wenliang, Zheng Zhuanzhen, Duan Junfang, Wang Xiaoru, Wu Shirong, Wang Wei, Xu Lu, Han Shuguang, Qiao Zhenhua
机构信息
Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
Department of Hematology, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
出版信息
Int J Clin Exp Med. 2015 Jun 15;8(6):10143-51. eCollection 2015.
This study aimed to develop a specific and sensitive method for the rapid detection of NF-κB activity in pancreatic tissue. Male Wistar rats were randomly divided into two groups: (1) 16 rats in the acute pancreatitis (AP) group received retrograde injection of 5% sodium taurocholate (STC) into the biliopancreatic duct, and (2) 16 rats in the Control group received saline. NF-κB activation in rat pancreatic acinar cells was assessed by flow cytometry (FCM). We found that the NF-κB activity in the AP group significantly increased at 1.5 h (29.80%±7.83), had a peak at 3 h (65.17%±13.22), and then decreased gradually to 12 h time point, close to the level after 1.5 h stimulation of STC. The NF-κB activity of the Control group did not significantly vary at different time points (P>0.05). FCM is a specific and sensitive assay for the rapid detection of NF-κB activity in pancreatic tissue.
本研究旨在开发一种用于快速检测胰腺组织中NF-κB活性的特异且灵敏的方法。雄性Wistar大鼠被随机分为两组：（1）急性胰腺炎（AP）组的16只大鼠接受逆行向胆胰管注射5%牛磺胆酸钠（STC），（2）对照组的16只大鼠接受生理盐水注射。通过流式细胞术（FCM）评估大鼠胰腺腺泡细胞中的NF-κB激活情况。我们发现，AP组中NF-κB活性在1.5小时时显著升高（29.80%±7.83），在3小时时达到峰值（65.17%±13.22），然后逐渐下降至12小时时间点，接近STC刺激1.5小时后的水平。对照组的NF-κB活性在不同时间点无显著变化（P>0.05）。流式细胞术是一种用于快速检测胰腺组织中NF-κB活性的特异且灵敏的检测方法。
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