Wang Yuan-Yu, Li Li, Ye Zai-Yuan, Zhao Zhong-Sheng, Yan Zhi-Long
Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, People's Republic of China.
Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou, 310014, Zhejiang, People's Republic of China.
World J Surg Oncol. 2015 Aug 28;13:259. doi: 10.1186/s12957-015-0673-8.
This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.
The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.
miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.
miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.
本研究旨在探讨miR-10b过表达对癌细胞增殖、迁移、侵袭及Hoxd10表达的影响。
转染miR-10b抑制剂或阴性对照B后，检测miR-10b对MKN-28、BGC-823和SGC-7901细胞增殖、迁移和侵袭的影响以及SGC-7901和BGC-823细胞中Hoxd10蛋白的表达。还研究了436例石蜡包埋癌组织中Hoxd10蛋白的表达。
miR-10b在AGS、MKN-28、BGC-823、HCG-27、SGC-7901和MKN-45细胞系中显著上调，转染48小时后，miR-10b抑制剂显著抑制MKN-45、BGC-823和SGC-7901细胞的增殖和迁移，而这些细胞系中的Hoxd10蛋白在转染72小时后有所增加。Hoxd10在胃癌中高表达，与肿瘤大小、Lauren分型、浸润深度、淋巴结及远处转移、肿瘤-淋巴结-转移（TNM）分期及预后相关。
miR-10b通过Hoxd10促进人胃癌细胞系的迁移和侵袭，可能在肿瘤发生、发展及预后中起重要作用。
