Langereis Martijn A, Rabouw Huib H, Holwerda Melle, Visser Linda J, van Kuppeveld Frank J M
Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
J Virol. 2015 Nov;89(21):11169-73. doi: 10.1128/JVI.01781-15. Epub 2015 Aug 26.
It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.
众所周知，在病毒感染之前进行质粒DNA转染会对感染效率产生负面影响。在此，我们表明胞质质粒DNA激活cGAS/STING信号通路，最终导致细胞抗病毒状态的诱导。使用瞬时单质粒成簇规律间隔短回文重复序列（CRISPR）/Cas9系统，我们生成了cGAS/STING基因敲除细胞，并表明这些细胞在质粒DNA转染后能够像未转染细胞一样高效地被感染。
