Lara Viviane de Paula Lima, Cardoso Fabiano Pereira, Brito Luciana Carla Neves, Vieira Leda Quercia, Sobrinho Antônio Paulino Ribeiro, Rezende Taia Maria Berto
Dental School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
Dental School, Universidade Itaúna, Itaúna, MG, BR.
Braz Dent J. 2015 Jul-Aug;26(4):337-41. doi: 10.1590/0103-6440201300006.
The aim of this study was to evaluate mineral trioxide aggregate (MTA)-induced cytokine expression in mice after experimental furcal perforation. BALB/c mice (n=5) were subjected to induced furcal drilling of the maxillary first molar followed by MTA sealing in the left side (experimental group) and paraffin sealing in the right side (control group). Animals were euthanized at 7, 14 and 21 days after sealing the perforations. The expression levels of the IFN-γ, TNF-α, IL-10, IL-4, TGF-β and RANKL genes were investigated by real time polymerase chain reaction (PCR) in the teeth and surrounding tissues. In the experimental groups, after the 7th day, there was a down-regulation of the mRNA levels of TNF-α and IL-4 compared to the 14th day (p<0.05). In these groups, the mRNA levels of RANKL, IFN-γ and TNF-α were statistically higher after 14 days compared to 21 days post-MTA sealing (p<0.05). The level of IL-10 mRNA was increased at the 21st day (p<0.05). The mRNA expression of TGF-β did not exhibit any statistically relevant results. There was a statistical down-regulation of IL-4 gene expressions when control and experimental groups were compared at days 7 and 21. In conclusion, MTA sealing favored the expression of pro-inflammatory cytokines in the intermediate phase of the immuno-inflammatory response (14th day). The reduction of these cytokines in later phase of the response was probably due to immunoregulation by IL-10.
本研究的目的是评估实验性根分叉穿孔后小鼠体内三氧化矿物凝聚体（MTA）诱导的细胞因子表达。将BALB/c小鼠（n = 5）的上颌第一磨牙进行诱导性根分叉钻孔，然后在左侧用MTA封闭（实验组），右侧用石蜡封闭（对照组）。在封闭穿孔后第7、14和21天对动物实施安乐死。通过实时聚合酶链反应（PCR）研究牙齿及周围组织中IFN-γ、TNF-α、IL-10、IL-4、TGF-β和RANKL基因的表达水平。在实验组中，第7天后，与第14天相比，TNF-α和IL-4的mRNA水平下调（p<0.05）。在这些组中，MTA封闭后14天的RANKL、IFN-γ和TNF-α的mRNA水平在统计学上高于21天（p<0.05）。IL-10 mRNA水平在第21天升高（p<0.05）。TGF-β的mRNA表达未呈现任何统计学相关结果。在第7天和第21天比较对照组和实验组时，IL-4基因表达存在统计学下调。总之，MTA封闭有利于在免疫炎症反应的中间阶段（第14天）促炎细胞因子的表达。反应后期这些细胞因子的减少可能是由于IL-10的免疫调节作用。
