牙髓病治疗材料对间充质干细胞的反应。

The response of Mesenchymal Stem Cells to endodontic materials.

机构信息

Department of Operative Denstistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Department of Operative Denstistry, School of Dentistry, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.

出版信息

Braz Dent J. 2022 Mar-Apr;33(2):33-43. doi: 10.1590/0103-6440202204786.

DOI:10.1590/0103-6440202204786

PMID:35508034

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9645149/

Abstract

An endodontic material must be minimally harmful to stem cells since they are essential, thanks to their capacity for cell proliferation, self-renewal, and differentiation. For this reason, in this in vitro study, the cell viability and the expression of genes involved in cell plasticity and differentiation were investigated in stem cells recovered from human dental pulp (hDPSCs) that were in contact with four endodontic materials (Endofill, MTA, Pulp Canal Sealer, and Sealer 26). The viability of HDPSCs was assessed by MTT and trypan blue exclusion assays. PCR evaluated cellular plasticity by determining the CD34, CD45, Nestin, CD105, Nanog, and OCT4 expressions. The effect on cell differentiation was determined by RT-PCR expression of the RUNX2, ALP, OC/BGLAP, and DMP1 genes. The data were analyzed using ANOVA with Bonferroni correction (p <0.05). Pulp Canal Sealer and Endofill decreased cell viability after 48 hours (p <0.001). MTA and Sealer 26 did not disrupt cell viability (p> 0.05). When cultivated in the presence of MTA and Sealer 26, hDPSCs expressed Nestin, CD105, NANOG, and OCT-4 and did not express CD34 and CD45. MTA and Sealer 26 interfered with DMP1, OC/BGLAP and RUNX2 expressions (p <0.05) but did not change ALP gene expression (p> 0.05). MTA and Sealer 26 showed biological compatibility in the presence of hDPSCs.

摘要

一种牙髓学材料必须对干细胞的危害最小，因为它们是必不可少的，这要归功于它们的细胞增殖、自我更新和分化能力。出于这个原因，在这项体外研究中，研究了接触四种牙髓学材料（Endofill、MTA、Pulp Canal Sealer 和 Sealer 26）后从人牙髓干细胞（hDPSCs）中回收的干细胞的细胞活力和参与细胞可塑性和分化的基因表达。通过 MTT 和台盼蓝排除试验评估 hDPSCs 的活力。PCR 通过确定 CD34、CD45、Nestin、CD105、Nanog 和 OCT4 的表达来评估细胞的可塑性。通过 RUNX2、ALP、OC/BGLAP 和 DMP1 基因的 RT-PCR 表达来确定对细胞分化的影响。使用具有 Bonferroni 校正的 ANOVA 分析数据（p<0.05）。Pulp Canal Sealer 和 Endofill 在 48 小时后降低细胞活力（p<0.001）。MTA 和 Sealer 26 未破坏细胞活力（p>0.05）。当在 MTA 和 Sealer 26 的存在下培养时，hDPSCs 表达 Nestin、CD105、NANOG 和 OCT-4，并且不表达 CD34 和 CD45。MTA 和 Sealer 26 干扰 DMP1、OC/BGLAP 和 RUNX2 的表达（p<0.05），但不改变 ALP 基因的表达（p>0.05）。MTA 和 Sealer 26 在存在 hDPSCs 时表现出生物相容性。