[脊椎动物线粒体DNA的简易分离方法]
[Simple methods of isolation of mitochondrial DNA in vertebrates].
作者信息
Brykov V A, Ivanov O A, Ivanova L N
出版信息
Ukr Biokhim Zh (1978). 1989 Nov-Dec;61(6):98-102.
PMID:2631328
Abstract
Two rapid and simple methods for isolating DNA from certain species of vertebrates are approbated. The both methods exclude the stage of centrifugation in the gradient of cesium chloride. The isolated DNA is hydrolyzed specifically by restricted endonucleases. Applicability of the mentioned methods is shown.
摘要
两种从某些脊椎动物物种中分离DNA的快速简单方法得到认可。这两种方法都省去了在氯化铯梯度中离心的步骤。分离出的DNA可被限制性内切酶特异性水解。展示了上述方法的适用性。
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