Increasing voltage gradient electrophoresis of DNA.
作者信息
Oliva Daniele, Di Maio Sabina, Sisino Giorgia, Bellavia Daniele, Barbieri Rainer
机构信息
Istituto di Biomedicina e Immunologia Molecolare del CNR, Palermo, Italy.
出版信息
J Chromatogr A. 2008 Apr 11;1187(1-2):205-8. doi: 10.1016/j.chroma.2008.02.033. Epub 2008 Feb 14.
We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.
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