Kumal Raju R, Landry Corey R, Abu-Laban Mohammad, Hayes Daniel J, Haber Louis H
Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Langmuir. 2015 Sep 15;31(36):9983-90. doi: 10.1021/acs.langmuir.5b02199. Epub 2015 Sep 3.
Photoactivated drug delivery systems using gold nanoparticles provide the promise of spatiotemporal control of delivery that is crucial for applications ranging from regenerative medicine to cancer therapy. In this study, we use second harmonic generation (SHG) spectroscopy to monitor the light-activated controlled release of oligonucleotides from the surface of colloidal gold nanoparticles. MicroRNA is functionalized to spherical gold nanoparticles using a nitrobenzyl linker that undergoes photocleaving upon ultraviolet irradiation. The SHG signal generated from the colloidal nanoparticle sample is shown to be a sensitive probe for monitoring the photocleaving dynamics in real time. The photocleaving irradiation wavelength is scanned to show maximum efficiency on resonance at 365 nm, and the kinetics are investigated at varying irradiation powers to demonstrate that the nitrobenzyl photocleaving is a one-photon process. Additional characterization methods including electrophoretic mobility measurements, extinction spectroscopy, and fluorimetry are used to verify the SHG results, leading to a better understanding of the photocleaving dynamics for this model oligonucleotide therapeutic delivery system.
使用金纳米颗粒的光活化药物递送系统有望实现时空可控的递送，这对于从再生医学到癌症治疗等一系列应用至关重要。在本研究中，我们使用二次谐波产生（SHG）光谱来监测寡核苷酸从胶体金纳米颗粒表面的光活化控释。使用硝基苄基连接子将微小RNA功能化到球形金纳米颗粒上，该连接子在紫外线照射下会发生光裂解。胶体纳米颗粒样品产生的SHG信号被证明是实时监测光裂解动力学的灵敏探针。扫描光裂解照射波长以显示在365 nm共振时的最大效率，并在不同照射功率下研究动力学，以证明硝基苄基光裂解是一个单光子过程。使用包括电泳迁移率测量、消光光谱和荧光测定法在内的其他表征方法来验证SHG结果，从而更好地理解该模型寡核苷酸治疗递送系统的光裂解动力学。
