Adamakis Ioannis Dimosthenis S, Panteris Emmanuel, Eleftheriou Eleftherios P
a Department of Botany ; School of Biology, Aristotle University ; Thessaloniki , Greece.
Plant Signal Behav. 2015;10(10):e1064572. doi: 10.1080/15592324.2015.1064572. Epub 2015 Aug 27.
Tungsten, supplied as sodium tungstate, inhibits root elongation in Arabidopsis thaliana, which has been attributed to a diminishing of PIN2 and PIN3 auxin efflux carriers. In this work, we sought to analyze the effect of tungsten on cortical microtubules and CLASP (Cytoplasmic Linker Associated Protein), which are also involved in the anisotropic cell expansion of root cells. Seedlings grown in a tungsten-free substrate for 4 d and then transplanted into a tungsten-containing substrate exhibited randomly oriented microtubules in a time-dependent manner. While tungsten had no effect on roots treated for 3 h, microtubule alignment was obviously affected in the transition and elongation zones after a 6, 12, 24, 48 h tungsten treatment, at prolonged tungsten administrations and in seedlings grown directly in the presence of tungsten. This change in microtubule orientation may be associated with the reduction of CLASP protein expression induced by tungsten, as evidenced in experiments with plants expressing the CLASP-GFP protein. A possible mechanism, by which the coordinated functions of CLASP, PIN2 and microtubules are affected, as revealed by inhibited root growth, is discussed.
以钨酸钠形式提供的钨会抑制拟南芥的根伸长，这归因于PIN2和PIN3生长素外排载体的减少。在这项工作中，我们试图分析钨对皮层微管和CLASP（细胞质连接相关蛋白）的影响，它们也参与根细胞的各向异性细胞扩张。在无钨基质中生长4天然后移植到含钨基质中的幼苗，其微管呈现出随时间变化的随机取向。虽然钨对处理3小时的根没有影响，但在经过6、12、24、48小时的钨处理后，在过渡区和伸长区，在长时间施用钨的情况下以及在直接在钨存在下生长的幼苗中，微管排列明显受到影响。微管取向的这种变化可能与钨诱导的CLASP蛋白表达减少有关，这在表达CLASP-GFP蛋白的植物实验中得到了证明。本文讨论了一种可能的机制，即通过抑制根生长揭示出CLASP、PIN2和微管的协同功能受到影响。
