Tokarev Y S, Voronin V N, Senderskiy I V, Issi I V
Parazitologiia. 2015 Mar-Apr;49(2):81-92.
The microsporidium Glugea gasterostei from the three-spined stickleback Gasterosteus aculeatus was described as an independent species basing upon morphological and ecological traits of the parasite (Voronin, 1974), further supported by ultrastructural characters of its spores (Voronin, 1983). During the revision of microsporidia of the genus Glugea (Canning, Lom, 1986; Lom, 2002), the validity of this species was doubted and it was synonymized with G. anomala. Nevertheless, the molecular phylogenetic analysis performed in the present study showed the unique molecular haplotype of small subunit rRNA gene of G. gasterostei (Genbank accession number KM977990) and its close relatedness to G. anomala, G. atherinae and G. hertwigi (sequence similarity of 99.7 %). One of typical characters of G. gasterostei, as opposed to G. anomala, is the formation of xenomas on inner tissues and not on the surface of infected fishes. This feature is retained even after the infection of different host species. Taken together, these data confirm the validity of G. gasterostei as a separate species among closely related taxa that had diverged comparatively recently.
基于寄生虫的形态和生态特征，来自三刺鱼（Gasterosteus aculeatus）的微孢子虫（Glugea gasterostei）被描述为一个独立的物种（沃罗宁，1974年），其孢子的超微结构特征进一步支持了这一观点（沃罗宁，1983年）。在对Glugea属微孢子虫进行修订期间（坎宁、洛姆，1986年；洛姆，2002年），该物种的有效性受到质疑，并被同义化为异形Glugea（G. anomala）。然而，本研究进行的分子系统发育分析显示，三刺鱼微孢子虫小亚基rRNA基因具有独特的分子单倍型（Genbank登录号KM977990），并且与异形Glugea、海鲱微孢子虫（G. atherinae）和赫氏微孢子虫（G. hertwigi）密切相关（序列相似性为99.7%）。与异形Glugea不同，三刺鱼微孢子虫的一个典型特征是在受感染鱼类的内部组织而非表面形成包囊瘤。即使在感染不同宿主物种后，这一特征仍然保留。综上所述，这些数据证实了三刺鱼微孢子虫作为一个独立物种在相对较近分化的密切相关分类群中的有效性。
