Vieira Larissa Rezende, Polomé Andy, Mesquita Rafael Dias, Salmon Didier, Braz Glória Regina Cardoso, Bousbata Sabrina
Proteomic Platform, Laboratory of Molecular Parasitology, Université Libre de Bruxelles, Belgium.
Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Brazil.
Proteomics. 2015 Nov;15(22):3901-4. doi: 10.1002/pmic.201400472. Epub 2015 Oct 19.
Rhodnius prolixus is an important vector of Trypanosoma cruzi, the causative agent of Chagas' disease, an illness that affects 20% of Latin America population. The obligatory course of the parasite in the vector digestive tract has made it an important target for investigation in order to control the parasite transmission and thus interrupt its biological cycle in the insect vector. Therefore, an insight into the vector midgut physiology is valuable for insect control as well as to provide potential novel targets for drugs and vaccines development and thus disease treatment. In this study, the first 2DE map of R. prolixus anterior midgut is described. Proteins were separated by 2DE and analyzed by nano-LC MS/MS. The results yielded 489 proteins from 475 spots. These proteins were classified into 28 functional groups and their physiological roles in the insect midgut are discussed. All MS data have been deposited in the ProteomeXchange with identifiers PXD001488 and PXD001489 (http://proteomecentral.proteomexchange.org/dataset/PXD001488, http://proteomecentral.proteomexchange.org/dataset/PXD001489).
克氏锥蝽是克氏锥虫的重要传播媒介，克氏锥虫是恰加斯病的病原体，这种疾病影响着20%的拉丁美洲人口。寄生虫在传播媒介消化道中的必经过程使其成为控制寄生虫传播从而中断其在昆虫传播媒介中生物循环的重要研究目标。因此，深入了解传播媒介中肠生理学对于昆虫控制以及为药物和疫苗开发提供潜在的新靶点从而治疗疾病具有重要价值。在本研究中，描述了克氏锥蝽前肠的首张二维电泳图谱。蛋白质通过二维电泳分离，并通过纳升液相色谱串联质谱进行分析。结果从475个斑点中鉴定出489种蛋白质。这些蛋白质被分为28个功能组，并讨论了它们在昆虫中肠的生理作用。所有质谱数据已存入蛋白质组交换库，标识符为PXD001488和PXD001489（http://proteomecentral.proteomexchange.org/dataset/PXD001488，http://proteomecentral.proteomexchange.org/dataset/PXD001489）。
