姜黄素对CD34(+)急性髓系白血病细胞增殖的抑制作用及其机制
[Inhibitory Effect of Curcumin on Proliferation of CD34(+) Acute Myeloid Leukemia Cells and Its Mechanism].
作者信息
Rao Jia, Zhang Rong-Yan, Chen Guo-An, Li Fei, Huang Ren-Wei
机构信息
Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; . E-mail:
Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):1005-8. doi: 10.7534/j.issn.1009-2137.2015.04.018.
OBJECTIVE
To explore the inhibitory effect of curcumin on proliferation of CD34(+) acute myeloid leukemia cells and its mechamism.
METHODS
KG1a and Kasumi-1cell lines were treated with curcumin of different concentrations (0, 40, 60, 80 µmol/L). The effect of curcumin on cell viability and proliferation was detected by trypan blue staining and cell count. The effect of curcumin on distribution of NF-κB P65 subunit was analyzed by immunofluorescence and Western blot.
RESULTS
The curcumin inhibited proliferation of KG1a and Kasumi-1 cells in a dose-dependent manner. Western blotting showed that curcumin led to significant down-regulation of NF-κB P65 nuclear protein expression. Immunofluorescence assay showed that treatment with 40 µmol/L of curcumin for 48h suppressed the nuclear translocation of NF-κB p65 in KG1a and Kasumi-1 cells.
CONCLUSION
The curcumin suppresses cell growth of KG1a and Kasumi-1 cells, its mechanism may be related to inhibitory effect of curcumin on NF-κB p65 nucleus protein.
目的
探讨姜黄素对CD34(+)急性髓系白血病细胞增殖的抑制作用及其机制。
方法
用不同浓度（0、40、60、80μmol/L）的姜黄素处理KG1a和Kasumi-1细胞系。通过台盼蓝染色和细胞计数检测姜黄素对细胞活力和增殖的影响。通过免疫荧光和蛋白质印迹法分析姜黄素对NF-κB P65亚基分布的影响。
结果
姜黄素以剂量依赖方式抑制KG1a和Kasumi-1细胞的增殖。蛋白质印迹法显示，姜黄素导致NF-κB P65核蛋白表达显著下调。免疫荧光分析显示，用40μmol/L姜黄素处理48小时可抑制KG1a和Kasumi-1细胞中NF-κB p65的核转位。
结论
姜黄素抑制KG1a和Kasumi-1细胞的生长，其机制可能与姜黄素对NF-κB p65核蛋白的抑制作用有关。
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