Hui Wu-Han, Ye Fei, Zhang Wei, Liu Cong-Yan, Xu Juan
Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):1062-8. doi: 10.7534/j.issn.1009-2137.2015.04.031.
To screen signaling pathway proteins in myelodysplastic/myeloproliferative neoplasms-unclassifiable (MDS/MPN-U), and to explore the possible role of the differentially expressed signaling pathway proteins in pathogenesis of MDS/MPN-U.
Protein Pathway Array (PPA) was applied to analyze the differential expression levels of signaling pathway proteins in 10 patients with MDS/MPN-U and normal controls, and furthermore to identify the signaling pathways and network in which these proteins were analyzed by Ingenuity pathway analysis program.
The expressions of 25 signaling proteins in MDS/MPN-U were significantly different, compared with the control group. Among them 15 proteins were upregulated in MDS/MPN-U patients, while 10 proteins were downregulated. These dysregulated proteins were involved in 10 major signaling pathways related with cell proliferation and immunity. The complicated interactive network was established by these proteins and pathways.
The differentially expressed signaling proteins screened from the MDS/MPN-U patients by PPA might be helpful to reveal the pathogenesis of MDS/MPN-U and to discover the therapeutic targets.
筛选骨髓增生异常/骨髓增殖性肿瘤，不可分类型（MDS/MPN-U）中的信号通路蛋白，并探讨差异表达的信号通路蛋白在MDS/MPN-U发病机制中的可能作用。
应用蛋白质通路阵列（PPA）分析10例MDS/MPN-U患者及正常对照中信号通路蛋白的差异表达水平，并通过Ingenuity通路分析程序进一步鉴定这些蛋白所在的信号通路及网络。
与对照组相比，MDS/MPN-U中25种信号蛋白的表达存在显著差异。其中15种蛋白在MDS/MPN-U患者中上调，10种蛋白下调。这些失调的蛋白参与了10条与细胞增殖和免疫相关的主要信号通路。这些蛋白和通路构成了复杂的交互网络。
通过PPA从MDS/MPN-U患者中筛选出的差异表达信号蛋白可能有助于揭示MDS/MPN-U的发病机制并发现治疗靶点。
