Duan Yafei, Li Jitao, Zhang Zhe, Li Jian, Ge Qianqian, Liu Ping
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao 266071, PR China.
Fish Shellfish Immunol. 2015 Dec;47(2):1067-74. doi: 10.1016/j.fsi.2015.08.018. Epub 2015 Aug 24.
Oncoprotein NM23, as a family of genes encoding the nucleoside diphosphate (NDP) kinase, plays important roles in bioenergetics, DNA replication, differentiation and tumor metastasis. In this study, a full-length cDNA of NM23 (designated EcNM23) was cloned from Exopalaemon carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EcNM23 was 755 bp, which contains an open reading frame (ORF) of 518 bp, encoding a 175 amino-acid polypeptide with the predicted molecular weight of 19.60 kDa and estimated isoelectric point of 7.67. The deduced amino acid sequence of EcNM23 shared high identity (86%-93%) with that of other crustaceans. a NDP kinase super family signature was identified in E. carinicauda EcNM23. Quantitative real-time RT-qPCR analysis indicated that EcNM23 was expressed in all the examined tissues with the high expression level in hemocytes and ovary. The EcNM23 expression in immune-related tissues changed rapidly and reached peak at different time after pathogens (Vibrio parahaemolyticus and WSSV) challenge and ammonia-N stress treatment. The results suggested that EcNM23 might be associated with the immune defenses to pathogens infection and ammonia-N stress in E. carinicauda.
癌蛋白NM23作为一个编码核苷二磷酸（NDP）激酶的基因家族，在生物能量学、DNA复制、细胞分化和肿瘤转移中发挥着重要作用。在本研究中，通过使用cDNA末端快速扩增（RACE）方法，从中华绒螯蟹中克隆出NM23的全长cDNA（命名为EcNM23）。EcNM23的全长cDNA为755 bp，其中包含一个518 bp的开放阅读框（ORF），编码一个175个氨基酸的多肽，预测分子量为19.60 kDa，估计等电点为7.67。EcNM23推导的氨基酸序列与其他甲壳类动物的氨基酸序列具有高度同源性（86%-93%）。在中华绒螯蟹EcNM23中鉴定出一个NDP激酶超家族特征。实时定量RT-qPCR分析表明，EcNM23在所有检测组织中均有表达，在血细胞和卵巢中表达水平较高。在病原体（副溶血性弧菌和WSSV）攻击和氨氮胁迫处理后的不同时间，免疫相关组织中的EcNM23表达迅速变化并达到峰值。结果表明，EcNM23可能与中华绒螯蟹对病原体感染和氨氮胁迫的免疫防御有关。
