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鉴定幽门螺杆菌耐药性的分子方法

Molecular Approaches to Identify Helicobacter pylori Antimicrobial Resistance.

作者信息

Mégraud Francis, Bénéjat Lucie, Ontsira Ngoyi Esther Nina, Lehours Philippe

机构信息

Bacteriology Laboratory, INSERM U853, University of Bordeaux, Bordeaux F-33000, France.

Bacteriology Laboratory, INSERM U853, University of Bordeaux, Bordeaux F-33000, France.

出版信息

Gastroenterol Clin North Am. 2015 Sep;44(3):577-96. doi: 10.1016/j.gtc.2015.05.002. Epub 2015 Jul 23.

Abstract

Antimicrobial susceptibility testing is needed to adapt Helicobacter pylori treatment to obtain the best results. Beside the standard phenotypic methods, molecular methods are increasingly used. The value of these molecular tests is that they are quick, independent of the transport conditions, easy to standardize, and commercial kits are available. In this article, these methods are reviewed, focusing on the determination of H pylori resistance to macrolides and fluoroquinolones, and mentioning also the methods used for tetracycline and rifampin.

摘要

为了使幽门螺杆菌治疗取得最佳效果,需要进行抗菌药敏试验。除了标准的表型方法外,分子方法的使用也越来越多。这些分子检测的价值在于它们速度快、不受运输条件影响、易于标准化,并且有商业试剂盒可供使用。在本文中,将对这些方法进行综述,重点关注幽门螺杆菌对大环内酯类和氟喹诺酮类药物的耐药性测定,同时也提及用于四环素和利福平的方法。

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