Chisholm Stephanie A, Owen Robert J
Gastrointestinal, Emerging, and Zoonotic Infections Department, Centre for Infections, Health Protection Agency, London NW9 5HT, UK.
Diagn Microbiol Infect Dis. 2008 May;61(1):67-71. doi: 10.1016/j.diagmicrobio.2007.12.005. Epub 2008 Jan 14.
The benefits of using a multiplex detection polymerase chain reaction (PCR) assay for Helicobacter pylori speciation and 2 real-time probe hybridization assays determining clarithromycin and tetracycline susceptibilities in gastric biopsies from 171 dyspeptic patients were investigated. Overall, 70 of 71 H. pylori culture-positive biopsies were PCR positive. For the 100 culture-negative biopsies, PCR identified a further 29 H. pylori positives (17% overall) and presence of resistance markers for clarithromycin (20/28) and tetracycline (2/28). The results demonstrated that PCR testing was valuable in providing improved detection rates and antibiotic susceptibility information when H. pylori culture was unsuccessful.
研究了使用多重检测聚合酶链反应（PCR）分析法进行幽门螺杆菌分型，以及两种实时探针杂交分析法测定171例消化不良患者胃活检组织中克拉霉素和四环素敏感性的益处。总体而言，71例幽门螺杆菌培养阳性的活检组织中有70例PCR检测呈阳性。对于100例培养阴性的活检组织，PCR又检测出29例幽门螺杆菌阳性（总体为17%），并检测出克拉霉素（20/28）和四环素（2/28）的耐药标志物。结果表明，当幽门螺杆菌培养未成功时，PCR检测对于提高检测率和提供抗生素敏感性信息具有重要价值。
