基于共轭聚合物-银纳米棱镜对的光学纳米标尺用于无标记蛋白质检测。

An optical nanoruler based on a conjugated polymer-silver nanoprism pair for label-free protein detection.

机构信息

State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R. China.

Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

出版信息

Adv Mater. 2015 Oct 21;27(39):6040-5. doi: 10.1002/adma.201502880. Epub 2015 Aug 28.

DOI:10.1002/adma.201502880

PMID:26314928

Abstract

An optical nanoruler system based on a conjugated polyelectrolyte-silver nanoprism pair is developed for label-free protein detection by taking advantage of the metal-enhanced fluorescence effect of silver nanostructures. Antibody-antigen interactions induce a change in the metal-fluorophore distance, followed by the response of a fluorescent signal of the conjugated polyelectrolyte. The system is used to detect target antigens sensitively and selectively.

摘要

一种基于共轭聚电解质-银纳米棱镜对的光学纳米标尺系统被开发出来，用于通过利用银纳米结构的金属增强荧光效应来进行无标记蛋白质检测。抗体-抗原相互作用引起金属荧光团距离的变化，随后共轭聚电解质的荧光信号发生响应。该系统可用于敏感和选择性地检测目标抗原。