Otta M E, Bertini F
Acta Physiol Lat Am. 1978;28(1):33-9.
Following a single intraperitoneal injection of oxonic acid, urate-oxidase inhibitor with optical and chromatographic properties of the injected drug was found in the supernatants of mouse liver homogenates. Maximal inhibitory activity of 40 000 g-20 minutes supernatants against Sigma urate-oxidase was found 30 minutes after injection, and it almost disappeared after six hours. In whole homogenates, urate-oxidase activity was found 50% inhibited at 30 minutes, and it returned to normal levels between 3 and 6 hours following injection. Urate-oxidase activity of resuspended nuclei-free 40 000 g particles from these homogenates showed a significative increase of activity over controls during the first three hours after injection, and it returned to a normal level between 3 and 6 hours after injection. There was, instead, no increase of activity in 40 000 g-20 minutes particles suspensions when these were separated from homogenates prepared from untreated mice and incubated with oxonic acid.
单次腹腔注射氧嗪酸后，在小鼠肝脏匀浆的上清液中发现了具有注射药物光学和色谱特性的尿酸氧化酶抑制剂。注射后30分钟，40000g - 20分钟上清液对Sigma尿酸氧化酶的最大抑制活性被发现，6小时后几乎消失。在整个匀浆中，注射后30分钟尿酸氧化酶活性被发现受到50%的抑制，注射后3至6小时恢复到正常水平。这些匀浆中重悬的无核40000g颗粒的尿酸氧化酶活性在注射后的前三小时内显示出比对照有显著的活性增加，并在注射后3至6小时恢复到正常水平。相反，当从未处理小鼠制备的匀浆中分离出40000g - 20分钟颗粒悬浮液并用氧嗪酸孵育时，其活性没有增加。
