Palombo F, Calcagnile A, Dogliotti E
Ann Ist Super Sanita. 1989;25(4):557-61.
The use of shuttle vectors has been applied in recent years to develop a better understanding of the molecular mechanisms of mutagenesis in mammalian cells. These recombinant DNA molecules replicate together with the host eukaryotic cells and can be retrieved in bacteria for rapid detection and analysis of mutation. Two approaches based on the use of shuttle vectors for studying the mutagenic effects of DNA lesions induced by alkylating agents are presented.
近年来，穿梭载体的应用有助于更深入地了解哺乳动物细胞中诱变的分子机制。这些重组DNA分子与宿主真核细胞一起复制，并可在细菌中回收，以便快速检测和分析突变。本文介绍了两种基于使用穿梭载体研究烷基化剂诱导的DNA损伤诱变效应的方法。
