Kadegowda Anil K G, Wright Asher, Duckett Susan K
Department of Animal and Veterinary Sciences; Clemson University ; Clemson, SC USA.
Adipocyte. 2014 Dec 10;3(4):304-13. doi: 10.4161/adip.32112. eCollection 2014 Oct-Dec.
The objective was to determine the effect of nutritional milieu of isolated stromal vascular (SV) cells on proliferative capacity of preadipocytes, and adipogenic and lipogenic capacity in adipocytes in vitro. Proliferation of the preadipocytes increased over time with 48 and 72 h being greater than 24 h; however, preadipocytes from steers supplemented with corn (LC) had lower proliferation rates compared with those without corn grain supplementation (L) at 72 h. Adipocyte cultures isolated from LC group had higher mean diameter on d 4 and 6, and higher mean volume on d 0, 4, 6, and 12 of culture. Adipocytes from steers supplemented with corn grain (LC) had lower expression of key adipogenic genes during extended days in culture. The results show that prior nutritional treatment of the donor animal used to isolate SV cultures alters their proliferative, adipogenic, and lipogenic capacity in culture. These differences may be related to lower induction/expression of AP2 gene in the adipose cultures from corn supplemented group. Corn grain supplementation to steers grazing legumes could have stimulated more active adipogenic progenitor cells to differentiate, which would leave fewer behind in the SV pool for subsequent isolation.
目的是确定分离的基质血管（SV）细胞的营养环境对前脂肪细胞增殖能力以及体外脂肪细胞成脂和生脂能力的影响。前脂肪细胞的增殖随时间增加，48小时和72小时的增殖高于24小时；然而，在72小时时，与未补充玉米粒的阉牛（L）相比，补充玉米的阉牛（LC）的前脂肪细胞增殖率较低。从LC组分离的脂肪细胞培养物在培养的第4天和第6天平均直径较大，在培养的第0天、第4天、第6天和第12天平均体积较大。在延长的培养天数中，补充玉米粒的阉牛（LC）的脂肪细胞关键成脂基因表达较低。结果表明，用于分离SV培养物的供体动物先前的营养处理会改变其在培养中的增殖、成脂和生脂能力。这些差异可能与补充玉米组脂肪培养物中AP2基因较低的诱导/表达有关。给放牧豆科植物的阉牛补充玉米粒可能刺激了更多活跃的成脂祖细胞分化，这会使SV池中留下更少的细胞用于后续分离。
