Böttger E C
Medizinische Hochschule Hannover, Institut für Medizinische, Mikrobiologie, FRG.
Biotechniques. 1989 Oct;7(9):925-6, 928-9.
The current methods for subcloning entire cDNA libraries usually result in a significant portion of recombinants containing multiple inserts, since in most instances the inserts derived from the library to be subcloned are released as a bulk of self-ligatable DNA fragments. By use of an adaptor strategy, a method is presented to confer noncompatible ends to primarily self-ligatable inserts, resulting in efficient subcloning of entire libraries as single insert recombinants.
