Wang Jing, Ren Tongjun, Han Yuzhe, Zhao Yang, Liao Mingling, Wang Fuqiang, Jiang Zhiqiang
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China.
Environ Toxicol Pharmacol. 2015 Sep;40(2):535-40. doi: 10.1016/j.etap.2015.08.012. Epub 2015 Aug 12.
Three different diets amended with lead nitrate [Pb(NO3)2] (100, 500 and 1000mg Pb/kg dry weight) and a Pb-free control diet (1.03mg Pb/kg dry weight) were fed to sea cucumber (Apostichopus japonicus) for 30 days. The patterns of Pb accumulation over time were determined in various tissues (body wall, intestine and respiratory tree), as well as growth performance and antioxidant enzymes activities. Pb accumulation in body wall and intestine increased with time in all dietary Pb treatments. When fed the highest Pb diet, the body wall exhibited the greatest Pb burden (16.37mg Pb/kg tissue wet weight), while Pb content in the intestine (2.68mg Pb/kg tissue wet weight) and the respiratory tree (1.78mg Pb/kg tissue wet weight) were lower than Pb content in the body wall by day 30. The body weight gain (BWG), specific growth rate (SGR) and survival rate (SR) had not been affected by 30 days oral administration of Pb supplemented diet. However, the antioxidant enzymes activities [superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)] of test groups were lower than control group in body wall and malondialdehyde (MDA) concentration in the body wall was opposite after 30 days in sea cucumbers. In summary, this work reports toxic effects in sea cucumber, A. japonicus, after dietary exposure to Pb.
用硝酸铅[Pb(NO₃)₂]（100、500和1000毫克铅/千克干重）改良的三种不同饲料以及一种无铅对照饲料（1.03毫克铅/千克干重）投喂刺参（仿刺参）30天。测定了不同组织（体壁、肠道和呼吸树）中铅随时间的积累模式，以及生长性能和抗氧化酶活性。在所有饲料铅处理组中，体壁和肠道中的铅积累均随时间增加。投喂最高铅含量饲料时，体壁的铅负荷最大（16.37毫克铅/千克组织湿重），而到第30天时，肠道（2.68毫克铅/千克组织湿重）和呼吸树（1.78毫克铅/千克组织湿重）中的铅含量低于体壁中的铅含量。30天口服补充铅的饲料对体重增加（BWG）、特定生长率（SGR）和存活率（SR）没有影响。然而，30天后，刺参体壁中试验组的抗氧化酶活性[超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）]低于对照组，而体壁中丙二醛（MDA）浓度则相反。总之，这项研究报告了刺参在经饲料暴露于铅后的毒性效应。
