Hirai Hiroyuki, Firpo Meri, Kikyo Nobuaki
Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA; Division of Endocrinology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
Stem Cell Res. 2015 Sep;15(2):384-6. doi: 10.1016/j.scr.2015.08.005. Epub 2015 Aug 17.
It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.
在没有白血病抑制因子（LIF）的情况下，从诸如成纤维细胞等分化细胞中建立小鼠诱导多能干细胞（iPSC）即便不是不可能，也是非常困难的。我们通过修饰的Oct4以及Sox2、Klf4和c-Myc建立并维持了不依赖LIF的iPSC超过120天。这些iPSC将为研究LIF-Stat3信号通路在小鼠多能干细胞中的作用提供一种新工具。
