利用回收离子液体预处理的玉米秸秆水解液，通过丙酮丁醇梭菌DSM 13864生产生物丁醇。
Biobutanol production from corn stover hydrolysate pretreated with recycled ionic liquid by Clostridium saccharobutylicum DSM 13864.
作者信息
Ding Ji-Cai, Xu Guo-Chao, Han Rui-Zhi, Ni Ye
机构信息
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
出版信息
Bioresour Technol. 2016 Jan;199:228-234. doi: 10.1016/j.biortech.2015.07.119. Epub 2015 Aug 20.
In this study, corn stover (CS) hydrolysates, pretreated by fresh and recycled ionic liquid (IL) [Bmim][Cl], were utilized in butanol fermentation by Clostridium saccharobutylicum DSM 13864. An efficient CS pretreatment procedure using [Bmim][Cl] was developed, giving a glucose concentration of 18.7 g L(-1) using ten times recycled [Bmim][Cl], representing about 77% of that produced with fresh IL (24.2 g L(-1)). Fermentation of hydrolysate I (pretreated by fresh IL) resulted in 7.4 g L(-1) butanol with a yield of 0.21 g g total-sugar(-1) and a productivity of 0.11 g L(-1)h(-1), while 7.9 g L(-1) butanol was achieved in fermentation using hydrolysate II (pretreated by ten times reused IL) with similar levels of acetone and ethanol, as well as yield and productivity. This study provides evidence for the efficient utilization of IL in CS pretreatment for biobutanol fermentation.
在本研究中，用新鲜的和循环使用的离子液体（IL）[Bmim][Cl]预处理的玉米秸秆（CS）水解产物被用于丁酸梭菌DSM 13864的丁醇发酵。开发了一种使用[Bmim][Cl]的高效CS预处理程序，使用十次循环的[Bmim][Cl]时葡萄糖浓度为18.7 g L(-1)，约为使用新鲜IL（24.2 g L(-1)）时产生浓度的77%。水解产物I（用新鲜IL预处理）发酵产生7.4 g L(-1)丁醇，产率为0.21 g g总糖(-1)，生产力为0.11 g L(-1)h(-1)，而使用水解产物II（用十次重复使用的IL预处理）发酵时，丁醇产量达到7.9 g L(-1)，丙酮和乙醇水平相似，产率和生产力也相近。本研究为IL在CS预处理用于生物丁醇发酵中的高效利用提供了证据。
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