Fabbrocini Adele, D'Adamo Raffaele, Del Prete Francesco, Maurizio Daniela, Specchiulli Antonietta, Oliveira Luis F J, Silvestri Fausto, Sansone Giovanni
Consiglio Nazionale delle Ricerche, Istituto di Scienze Marine, UOS Lesina (FG), Italy.
Consiglio Nazionale delle Ricerche, Istituto di Scienze Marine, UOS Lesina (FG), Italy.
Ecotoxicol Environ Saf. 2016 Jan;123:53-9. doi: 10.1016/j.ecoenv.2015.08.018. Epub 2015 Aug 28.
Changes in environmental stressors inevitably lead to an increasing need for innovative and more flexible monitoring tools. The aim of this work has been the characterization of the motility pattern of the cryopreserved sea bream semen after exposure to a dumpsite leachate sample, for the identification of the best representative parameters to be used as endpoints in an ecotoxicological bioassay. Sperm motility has been evaluated either by visual and by computer-assisted analysis; parameters concerning motility on activation and those describing it in the times after activation (duration parameters) have been assessed, discerning them in terms of sensitivity, reliability and methodology of assessment by means of multivariate analyses. The EC50 values of the evaluated endpoints ranged between 2.3 and 4.5ml/L, except for the total motile percentage (aTM, 7.0ml/L), which proved to be the less sensitive among all the tested parameters. According to the multivariate analyses, a difference in sensitivity among "activation" endpoints in respect of "duration" ones can be inferred; on the contrary, endpoints seem to be equally informative either describing total motile sperm or the rapid sub-population, as well as the assessment methodology seems to be not discriminating. In conclusion, the CRYO-Ecotest is a multi-endpoint bioassay that can be considered a promising innovative ecotoxicological tool, characterized by a high plasticity, as its endpoints can be easy tailored each time according to the different needs of the environmental quality assessment programs.
环境压力源的变化不可避免地导致对创新且更灵活的监测工具的需求不断增加。这项工作的目的是表征冷冻保存的海鲷精液在暴露于垃圾填埋场渗滤液样品后的运动模式，以确定在生态毒理学生物测定中用作终点的最佳代表性参数。精子活力已通过视觉和计算机辅助分析进行评估；评估了有关激活时的活力参数以及描述激活后各时间段活力的参数（持续时间参数），并通过多变量分析从敏感性、可靠性和评估方法方面对它们进行了区分。除总活动率（aTM，7.0ml/L）外，评估终点的半数有效浓度（EC50）值在2.3至4.5ml/L之间，总活动率在所有测试参数中被证明是最不敏感的。根据多变量分析，可以推断出“激活”终点相对于“持续时间”终点在敏感性上存在差异；相反，无论是描述总活动精子还是快速亚群，终点似乎同样具有信息价值，而且评估方法似乎也没有区分度。总之，CRYO - 生态测试是一种多终点生物测定法，可被视为一种有前景的创新生态毒理学工具，具有高度可塑性，因为其终点可根据环境质量评估计划的不同需求轻松定制。
