Li Siming, Yang Xiaohong, Tang Shenghui, Zhang Xunmeng, Feng Zhencheng, Cui Shuliang
Guangzhou Institute of Traumatic Surgery, The Fourth Affiliated Hospital, Jinan University School of Medicine, 396 Tongfu Zhonglu Road, Haizhu District, Guangzhou, 510220, Guangdong Province, People's Republic of China,
J Mater Sci Mater Med. 2015 Aug;26(8):227. doi: 10.1007/s10856-015-5557-5. Epub 2015 Aug 30.
Surgical replacement of massively defected joints necessarily relies on osteochondral grafts effective to both of bone and cartilage. Demineralized bone matrix (DBM) retains the osteoconductivity but destroys viable chondrocytes in the cartilage portion essential for successful restoration of defected joints. This study prepared osteochondral grafts of DBM with protected cartilage. Protected cartilage portions was characterized by cellular and molecular biology and the grafts were allogenically used for grafting. Protected cartilage showed similar histomorphological structure and protected proteins estimated by total proteins and cartilage specific proteins as in those of fresh controls when DBMs were generated in bone portions. Such grafts were successfully used for simultaneously repair of bone and cartilage in massively defected osteoarticular joints within 16 weeks post-surgery. These results present an allograft with clinical potential for simultaneous restoration of bone and cartilage in defected joints.
对严重缺损关节进行手术置换必然依赖于对骨和软骨均有效的骨软骨移植物。脱矿骨基质（DBM）保留了骨传导性，但破坏了软骨部分中对成功修复缺损关节至关重要的活软骨细胞。本研究制备了具有保护软骨的DBM骨软骨移植物。通过细胞和分子生物学对保护的软骨部分进行了表征，并将移植物异体用于移植。当在骨部分生成DBM时，保护的软骨显示出与新鲜对照相似的组织形态结构，并且通过总蛋白和软骨特异性蛋白估计的保护蛋白也相似。此类移植物在术后16周内成功用于同时修复严重缺损的骨关节中的骨和软骨。这些结果表明一种同种异体移植物具有在缺损关节中同时修复骨和软骨的临床潜力。
