Salazar Gabriela, Vásquez Fabián, Rodríguez Maria P, Andrade Ana M, Anziani Maria A, Vio Fernando, Coward Williams
Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago (Chile)..
MRC Human Nutrition Research, Cambridge, United Kingdom..
Nutr Hosp. 2015 Sep 1;32(3):1067-74. doi: 10.3305/nh.2015.32.3.9263.
the doubly labelled water (DLW) method has an accuracy of 1% and within-subject precision of 5-8%, depending on subject's age and environments issues. Energy intake assessment is prone to errors (>15- 20%) depending in the method utilized.
to quantify DLW methodology errors in four to five year olds that could affect the comparison with energy intake.
energy expenditure (TEE, by DLW), was assessed during 14 days in 18 preschool children, who attended eight hours daily to day-care centres. Energy intake was determined by a combined method: food weighing during weekdays and recall after leaving the Centre (17h to sleep time) plus 24 h recall, during the weekend. Several assumptions affecting DLW total error were assessed to determine their influence in the comparison to energy intake (i.e. background variability, space ratio, proportion of water subject to fractionation, food quotient value).
the individual mean energy expenditure was 1 373 ± 177 kcal and the energy intake (1 409 ± 161 kcal). The overall difference between intake and expenditure was 42.9 kcal/day (limits of agreement + 259.1 to -112.3 kcal/day). TEE measurement error only explained a minor quantity (2.4%), between both measurements, and the observed mean isotope dilution space was 1.030 ± 0.010 confirming the value utilized in adults studies.
energy expenditure data is similar to other studies in preschool children. The small difference found between energy intake and expenditure may be attributed to the applied energy intake methodology, the homogeneous diet at care centres during the week-days and the lower DLW methodology error.
双标水（DLW）法的准确度为1%，受试者内精密度为5 - 8%，具体取决于受试者年龄和环境因素。能量摄入评估容易出现误差（>15 - 20%），这取决于所使用的方法。
量化可能影响与能量摄入比较的4至5岁儿童DLW方法误差。
对18名学龄前儿童进行了为期14天的能量消耗（通过DLW法测定总能量消耗，TEE）评估，这些儿童每天在日托中心待8小时。能量摄入通过一种组合方法确定：工作日食物称重，离开中心后（17时至睡眠时间）回忆加上周末24小时回忆。评估了几个影响DLW总误差的假设，以确定它们在与能量摄入比较中的影响（即背景变异性、空间比、分馏水的比例、食物商值）。
个体平均能量消耗为1373±177千卡，能量摄入为（1409±161千卡）。摄入与消耗之间的总体差异为42.9千卡/天（一致性界限为+259.1至 - 112.3千卡/天）。TEE测量误差仅解释了两次测量之间的一小部分差异（2.4%），观察到的平均同位素稀释空间为1.030±0.010，证实了成人研究中使用的值。
能量消耗数据与其他学龄前儿童研究相似。能量摄入与消耗之间的微小差异可能归因于所应用的能量摄入方法、工作日护理中心的同质化饮食以及较低的DLW方法误差。
