van Tienhoven-Wind Lynnda J N, Perton Frank G, Dullaart Robin P F
University of Groningen and University Medical Center Groningen, The Netherlands.
University of Groningen and University Medical Center Groningen, The Netherlands.
Clin Biochem. 2016 Jan;49(1-2):41-6. doi: 10.1016/j.clinbiochem.2015.08.024. Epub 2015 Aug 28.
Low-normal thyroid function within the euthyroid range may influence plasma lipoprotein levels. Associations between variation in thyroid function and pre-β-high density lipoproteins (pre-β-HDL), i.e. lipid-poor or lipid free HDL particles that act as initial acceptor of cell-derived cholesterol, are unknown. We determined relationships of plasma pre-β-HDL with thyroid function in euthyroid subjects with and without type 2 diabetes mellitus (T2DM).
TSH, free T4, plasma (apo)lipoproteins, pre-β-HDL, pre-β-HDL formation (pre-β-HDL generation during incubation with lecithin:cholesterol acyltransferase being inhibited) and phospholipid transfer protein (PLTP) activity were measured in fasting plasma from 72 T2DM and 82 non-diabetic subjects.
TSH was similar and free T4 was slightly higher (P < 0.05) in T2DM vs. non-diabetic subjects. HDL cholesterol and apoA-I were lower, whereas pre-β-HDL (expressed as % of apoA-I), triglycerides and PLTP activity were higher in T2DM (P < 0.05 to P < 0.001). In T2DM, pre-β-HDL formation (in apoA-I concentration and in % of apoA-I) was positively related to free T4, PLTP activity, total cholesterol and triglycerides (P < 0.05 for each). Multivariable linear regression analyses, adjusted for age, sex, PLTP activity, total cholesterol and triglycerides, demonstrated that pre-β-HDL formation was positively related to free T4 (in apoA-I concentration: β = 0.278, P = 0.014; in % of apoA-I: β = 0.343, P = 0.003) in T2DM, but not in non-diabetic subjects (both P > 0.30; interaction terms: both P < 0.05).
Variations in thyroid function within the euthyroid range may influence the metabolism of pre-β-HDL in T2DM.
甲状腺功能正常范围内甲状腺功能略低可能会影响血浆脂蛋白水平。甲状腺功能变化与前β-高密度脂蛋白（pre-β-HDL）之间的关联尚不清楚，前β-高密度脂蛋白即作为细胞源性胆固醇初始受体的低脂或无脂高密度脂蛋白颗粒。我们确定了患有和未患有2型糖尿病（T2DM）的甲状腺功能正常受试者血浆前β-HDL与甲状腺功能之间的关系。
在72例T2DM患者和82例非糖尿病患者的空腹血浆中检测促甲状腺激素（TSH）、游离甲状腺素（free T4）、血浆（载脂蛋白）脂蛋白、前β-HDL、前β-HDL形成（在与卵磷脂：胆固醇酰基转移酶孵育期间前β-HDL生成受到抑制）以及磷脂转运蛋白（PLTP）活性。
与非糖尿病患者相比，T2DM患者的TSH相似，游离T4略高（P<0.05）。T2DM患者的高密度脂蛋白胆固醇和载脂蛋白A-I较低，而前β-HDL（以载脂蛋白A-I的百分比表示）、甘油三酯和PLTP活性较高（P<0.05至P<0.001）。在T2DM患者中，前β-HDL形成（以载脂蛋白A-I浓度和载脂蛋白A-I的百分比表示）与游离T4、PLTP活性、总胆固醇和甘油三酯呈正相关（每项P<0.05）。经年龄、性别、PLTP活性、总胆固醇和甘油三酯校正的多变量线性回归分析表明，T2DM患者中前β-HDL形成与游离T4呈正相关（以载脂蛋白A-I浓度表示：β=0.278，P=0.014；以载脂蛋白A-I的百分比表示：β=0.343，P=0.003），而在非糖尿病患者中则无此相关性（两者P>0.30；交互项：两者P<0.05）。
甲状腺功能正常范围内的甲状腺功能变化可能会影响T2DM患者前β-HDL的代谢。
